FABL1_16 Biotechnology of Drugs

Faculty of Pharmacy
Spring 2025
Extent and Intensity
3/0/0. 4 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (lecturer)
doc. RNDr. Jan Hošek, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jan Hošek, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Prerequisites
The knowledge of subject in range of lectures.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20
fields of study / plans the course is directly associated with
Course objectives
The course is demonstration of Pharmaceutical Biotechnology - scientific discipline focusing on all technologies required for the production, manufacturing and registration of biotechnological drugs. Students will be introduced to processes which are used by organisms or their parts to production or modification of drugs. The methods used for breeding of animals, plants and microorganisms usable in medicine and pharmacy will be discussed. The main focus is done for recombinant techniques, gene and protein engineering and gene therapy. Products of industrial biotechnology as antibiotics, vitamins, organic acids, amino acids and alkaloids, will be presented.
Learning outcomes
Basic knowledge from cytology, gene engineering and industrial microbiology usable in recombinant drugs design, development and production. View to biotechnological production of antibiotics, vitamins, organic acids, amino acids and alkaloids.
Syllabus
  • 1) Introduction to pharmaceutical biotechnology, definition, milestones
    2) The cell as a tool of biotechnology, function of endoplasmic reticulum, Golgi komplex, posttranslational processes, chaperones
    3) The basics of gene engineering I - the principles, how to prepare recombinant gene, vectors
    4) The basics of gene engineering II - preparation of genes for cloning, host cells, transformation, selection and transformants identification
    5) Expression of recombinant proteins in prokaryotic cells and yeasts
    6) Expression of recombinant proteins in eukaryotic cells - insect cells and baculoviruses, mammalian cells and adenoviruses
    7) The classical biotechnological processes in pharmacology
    8) The process of biotechnology - definition, the phases of biotechnological process, basic materials, fermentors and bioreactors, methods of biotechnological products purification
    9) Examples of classical and recombinant biotechnology products in pharmacy, cytokines, hormones, enzymes, antibodies and their derivates
    10) Gene engineering of plants - structure of plant genome, vectors, expression cassettes
    11) Plant biotechnology - methods of transformation and transgene identification, molecular pharming
    12) Application of gene engineering and plant biotechnology in pharmacy, medicine, and food industry, rules for genetically modified organisms manipulation
    13) Gene and cell therapy, tissue engineering, genomics and proteomics in pharmacy
    14) New trends in biopharmaceutical products development
Literature
    required literature
  • Brown, T.A. (2010). Gene cloning and DNA analysis, an introduction. 6th edition, Wiley-Blackwell. info
  • Alberts et al. (1998). Molecular Biology of the Cell, 5th edition, GS Garland Science. info
  • Walsh, G. (2007). Pharmaceutical Biotechnology, John Wiley and sons. info
  • HO, R. J. Y. , GIBALDI, M. Biotechnology and Biopharmaceuticals. Transforming proteins and genes into drugs. John Willey and Sons, Hobokey, New Jersey, U.S.A, 2003. info
  • SLATER, A., SCOTT, N., FOWLER, M. Plant biotechnology. The genetic manipulation of plants. Oxford University Press. Oxford, New York, U.S.A, 2003. info
    recommended literature
  • Alberts et al. Essential cell biology. Garland Science, Taylor and Francis Group. ISBN 0-8153-3481-8. info
    not specified
  • KAYSER, O., MÜLLER, R.H. Pharmaceutical Biotechnology. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim, Ge, 2004. info
Teaching methods
lectures, videos
Assessment methods
Written examination test and oral examination
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2020, Spring 2021, Spring 2024.
  • Enrolment Statistics (Spring 2025, recent)
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