B8312 Practical Course in Molecular Biology of Viruses

Faculty of Science
Spring 2002
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
RNDr. Jana Kailerová, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
B7311 Mol. biol. of procar.-practice
1. Basic methods in molecular biology 2. Molecular biology of DNA and RNA viruses 3. Life and reproduction of bacteriophages
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 0/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Preparation and cultivation of bacterial viruses; Preparation of phage lysate; Assessing of the phage titre ; One step growth curve of the bacteriophage; Assessing of the burst size of phages; Mutation index; Induction of the phage using UV-light; Transduction in Staphylococcus aureus; Isolation of the temperature sensitive mutants of bacteriophages
Syllabus
  • 1. One step growth curve of polyvalent phage 812 2. Preparing of ts mutant sensitive to high temperature. 3. Estimation of frequency of mutation in phages 4. Kinetics of adsorption, construction of adsorption curve 5. Induction of prophage, delysogenization in the host cells of S. aureus 6. Calculation the force of gene binding, estimation of distance between genes 7. Construstion of genomic map of phages using two- and three factor crossing 8. Estimation of burst-size of phage from single cells of S. aureus or E. coli using Poissons distribution.
Literature
  • Návody k cvičení jsou u vyučující
  • Virology methods manual. Edited by B. W. J. Mahy - Hillar O. Kangro. London: Academic Press, 1996, x, 374. ISBN 0124653308. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Spring 2000, Spring 2001.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2002/B8312