C7105 Biochemistry – metabolism

Faculty of Science
Autumn 2024
Extent and Intensity
2/1/0. 5 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Fri 12:00–13:50 C05/114, Fri 14:00–14:50 C05/114
Prerequisites
Basic knowledge of organic and general chemistry, biology
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The course covers the basic fields of biochemistry from the chemical point of view. Most important properties and structure of natural substances(aminoacids and proteins, carbohydrates, lipids) are described here. The general description of chemical reactions in the cell includes enzymes and coenzymes, their properties and structure, active site, thermodynamics, enzyme kinetics, inhibition, allostery and regulation of enzyme acitivity. Special chapters describe most important metabolism of carbohydrates and lipids with emphasis on energetic features of metabolism.
The goal of this course in to equip students with the basic knowledge about chemical principles of metabolism.
Learning outcomes
The goal of this course in to equip students with the basic knowledge about chemical principles of metabolism of saccharides and lipids, cellular bioenergetics, structure and function of proteins and enzyme catalysis.
Syllabus
  • 1. Aminoacids, properties, determination, chromatography 2. Peptides, peptide bond, primary, secondary, terciary, quaternary structure 3. Non-repetitive structures, colagene, hydropathy, properties of proteins 4. Glycides, properties, reactions, anomers, hemiacetals, anomers. conformation 5. Glycosides, oligosaccharides, polysaccharides (starch, glycogen, chitin), heteropolysaccharides. 6. Lipids, acylglycerols, phospholipids, plasmalogens, sphingolipids, steroids 7. Nucleic acids, nucleosides, nucleotides, primary structure 8. DNA, RNA, double-helix, superhelix, denaturation, hybrid structure, methods for determination of primary structure 9. Thermodynamics in biochemistry, macroergic bond, reaction kinetics, enzymes, their active site, prosthetic groups, coenzymes, mechanism of enzyme catalysis 10. Coenzymes and vitamins, nicotinamide and NAD, flavins, ATP. AMO, cAMO, biotin, thiamin, coenzyme A, lipoic acid, folic acid 11. Pyridoxalphosphate, B12, metaloporphyrins, iron-sulfur proteins, vitamin C, lipophilic vitamins 12. Michaelis-Menten equation, Km, VL, turnover number, enzyme activity 13. Anabolism, catabolism, their regulation, anaerobic glycolysis 14. Gluconeogenesis, synthesis of PEP, glycogenolysis, synthesis of glycogen, Cori cycle
Literature
  • Vodážka - Biochemie
  • Voet, Voetová - Biochemie; Zubay, Parson, Vance - Principles of Biochemistry
  • Mikeš - Základní pojmy z biochemie II
Teaching methods
Theoretical lecture.
Assessment methods
Lecture 2 hr. Written examination.
Language of instruction
English
Further comments (probably available only in Czech)
Information on completion of the course: Předmět se ukončuje prezenčně písemnou zkouškou, alternativně ve zdůvodněných případech distanční ústní zkouškou.
The course can also be completed outside the examination period.
The course is taught annually.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/C7105