PřF:B7120 Molecular biology of prokaryot

B7120 Molecular biology of prokaryotes

Faculty of Science
Autumn 1999

Extent and Intensity

3/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Jiří Doškař, CSc. (lecturer)

Guaranteed by

Prerequisites (in Czech)

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

Terminology of bacterial genetics. Structure and reproduction of bacterial chromosome. Plasmids (general characteristic of plasmid, plasmid-borne functions, regulation of plasmid replication, plasmid stability, plasmid incompatibility and recombination). F plasmid, R plasmids, Col plasmids, Ti plasmids of Agrobacterium tumefaciens. Prokaryotic transposons, genetics with transposons. Transposon mutagenesis. Conjugation (interrupted and non interrupted conjugation, properties of different Hfr strains, zygotic induction, F$^x $F$^-$ crosses). Transduction (general, specific and abortive transduction, crosses based on general transduction, characteristics of transducing phages). Transformation. Mutations of bacteria (auxotrophic mutants, mutants resistant to antibiotics, nuclear segregation, phenotypic delay, fluctuation test, indirect selection of mutants, mutation rate). DNA repair. Restriction and modification of DNA, RM systems of bacteria. Molecular taxonomy of prokaryotes.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

The course is also listed under the following terms Autumn 2000, Autumn 2001.