C3600 Biochemistry - laboratory course

Faculty of Science
Autumn 2002
Extent and Intensity
0/5/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Mgr. Jiří Mazoch, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
prof. RNDr. Michaela Wimmerová, Ph.D. (seminar tutor)
doc. RNDr. Petr Zbořil, CSc. (seminar tutor)
Guaranteed by
doc. Ing. Martin Mandl, CSc.
Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C3600/Cb29: No timetable has been entered into IS. P. Boublíková
C3600/Cb28: No timetable has been entered into IS. M. Mandl
C3600/Cb27: No timetable has been entered into IS. P. Skládal
Prerequisites (in Czech)
NOW( C3580 Biochemistry )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goals of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.
Syllabus
  • Qualitative reactions of carbohydrates. Determination of glucose using a biosensor. Carbohydrate thin-layer chromatography.
  • Isolation and identification of lipids from the egg yolk.
  • Protein electrophoresis of the blood serum. Determination and chromatographic separation of leaf pigments. Gel permeation chromatography in separation of biomolecules.
  • Chemical reactions of amino acids and proteins. Determination of amino acids and peptides. Paper chromatography of amino acids. Protein isolation.
  • Isolation and properties of nucleic acids.
  • Heat dependence of enzyme reaction.
  • Protein determination.
  • pH effect on enzyme reaction. Determination of the rate of enzyme reaction.
  • Substrate specifity of enzymes. Determination of enzyme activity.
  • Enzyme kinetics. Enzyme inhibition.
  • Enzymatic determination of metabolites.
  • Electron transport chain of aerobic organisms.
  • Closing test.
Literature
  • MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Assessment methods (in Czech)
První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2002, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2002/C3600