C7895 Mass Spectrometry of Biomolecules

Faculty of Science
Autumn 2002
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Chemistry Section – Faculty of Science
Prerequisites
Knowledge of Analytical Chemistry on a level of introductory course Knowledge of high school Physics.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Analytical Mass Spectrometry. The course will provide mass spectrometry basics: ionization methods, mass analyzers, ion detectors with a focus on mass spectrometry of biomolecules (ionization methods MALDI, ESI) and modern instrumentation in mass spectrometry (TOFMS, ion trap, FTMS).
Syllabus
  • 1. A Brief Historical Perspective: Overview of Techniques and Technology. Basic Concepts of MS (resolution, sensitivity). 2. Ionization Techniques and Sample Introduction: Electron Impact (EI). Chemical Ionization (CI). Glow Discharge. Inductively Coupled Plasma (ICP). Fast Atom Bombardment (FAB). Secondary Ion Mass Spectrometry (SIMS). Thermospray (TSI). Electrospray (ESI). Laser Desorption (LD). Plasma Desorption (PD). Matrix-Assisted Laser Desorption/Ionization (MALDI). Coupling of Separation and MS (on-line, off-line, chips) 3. Mass Spectrometers: Ion Optics. Simulation of Ion Movement (Simion). Energy Analyzers. Magnetic Sector. Quadrupole Mass Analyzer. Ion Cyclotron Resonance Fourier Transform Mass Spectrometer (FT-ICR-MS). Ion Trap (IT). Time-of-Flight Mass Spectrometer (TOFMS). Collision-Induced Dissociation (CID). Tandem Mass Spectrometry (MS/MS). Vacuum Principles & Techniques. Future of MS? Orbitrap. Detectors & Detection Electronics. 4. MS Applications: Proteins and peptides. Peptide mapping, protein databases. DNA. Saccharides. Synthetic polymers.
Literature
  • COTTER, Robert J. Time-of-Flight Mass Spectrometry: Instrumentation and applications in biological research. Washington, D.C.: American Chemical Society, 1997, 326 pp. ISBN 0-8412-3474-4. info
  • COLE, Richard B. Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation & Applications. John Wiley & Sons, Inc., 1997, 577 pp. ISBN 0-471-14564-5. info
Assessment methods (in Czech)
Přednášky, ústní zkouška. / Lectures, final oral examination.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
General note: FALL SEMESTER 2004: THE COURSE WILL BE GIVEN IN ENGLISH.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2001, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2002, recent)
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