C7895 Mass Spectrometry of Biomolecules

Faculty of Science
Autumn 2013
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Mgr. Iva Benešová, Ph.D. (assistant)
Christian Nilsson, PhD. (assistant)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Tue 17:00–18:50 B11/235
Prerequisites
Knowledge of Analytical Chemistry on a level of introductory course Knowledge of high school Physics. The course is given in Czech or English - as requested by students. The final oral examination will be in Czech or English.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Student will understand fundamentals of mass spektrometry including ionization methods, mass analyzers, ion detectors and selected applications, e.g. ionization mtechniques MALDI and ESI, modern mass snalyzers time-of-flight, ion traps and orbitrap, identification and quantification of proteins.
Syllabus
  • 1. A Brief Historical Perspective: Overview of Techniques and Technology. Basic Concepts of MS (resolution, sensitivity).
  • 2. Ionization Techniques and Sample Introduction: Electron Impact (EI). Chemical Ionization (CI). Glow Discharge. Inductively Coupled Plasma (ICP). Fast Atom Bombardment (FAB). Secondary Ion Mass Spectrometry (SIMS). Thermospray (TSI). Electrospray (ESI). Laser Desorption (LD). Plasma Desorption (PD). Matrix-Assisted Laser Desorption/Ionization (MALDI). Coupling of Separation and MS (on-line, off-line, chips)
  • 3. Mass Spectrometers: Ion Optics. Simulation of Ion Movement (Simion). Energy Analyzers. Magnetic Sector. Quadrupole Mass Analyzer. Ion Cyclotron Resonance Fourier Transform Mass Spectrometer (FT-ICR-MS). Ion Trap (IT). Linear Trap (LT). Orbitrap. Time-of-Flight Mass Spectrometer (TOFMS). Collision-Induced Dissociation (CID). Tandem Mass Spectrometry (MS/MS). Vacuum Principles & Techniques. Detectors & Detection Electronics.
  • 4. MS Applications: Proteins and peptides. Peptide mapping, protein databases. DNA. Saccharides. Synthetic polymers.
Literature
    recommended literature
  • COTTER, Robert J. Time-of-Flight Mass Spectrometry: Instrumentation and applications in biological research. Washington, D.C.: American Chemical Society, 1997, 326 pp. ISBN 0-8412-3474-4. info
  • COLE, Richard B. Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation & Applications. John Wiley & Sons, Inc., 1997, 577 pp. ISBN 0-471-14564-5. info
Teaching methods
Lectures and discussion in the end of the semester. Visit of a mass spectrometry laboratory, demonstrations of MS hardware.
Assessment methods
Final oral examination (in Czech or English). The exam consists of three questions on the following topics: 1. ionization techniques, 2. mass analyzers, 3. applications. Fifty percent of correct answers is needed to pass.
Language of instruction
English
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
General note: V podzimním semestru 2011 bude předmět vyučován v anglickém jazyce. Zkoušku je možné složit v češtině.
Teacher's information
http://bart.chemi.muni.cz/courses.htm
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2013, recent)
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