PřF:Bi7430 Molecular biotechnology - Course Information
Bi7430 Molecular biotechnology
Faculty of ScienceAutumn 2006
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer) - Guaranteed by
- doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science - Timetable
- Tue 14:00–15:50 Bpt,01013
- Prerequisites
- Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- Course objectives
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creating and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Directed mutagenesis and protein engineering. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
- Syllabus
- Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Selection of cells with recombinant DNA. Creation and screening of genomic libraries. Artificial gene transfer. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Direct mutagenesis and protein engineering.Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
- Literature
- Glick B.R., Pasternak J.J. 2003, Molecular Biotechnology, ASM Press, Washington, D.C.
- Assessment methods (in Czech)
- Předmět je ukončen zkouškou
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2006, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2006/Bi7430