Bi5710c Microbiology - practice

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Ludmila Tvrzová, Ph.D. (seminar tutor)
doc. Mgr. Monika Vítězová, Ph.D. (seminar tutor)
doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Guaranteed by
doc. RNDr. Miroslav Němec, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc.
Prerequisites (in Czech)
NOW( Bi5710 Microbiology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the practical course of general microbiology are basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples).
Syllabus
  • 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of Microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition 5. Determination of number of bacteria by cultivation method 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. Inoculation of microscopic fungi. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negativ - staining, capsule - staining. Microscopic examination of endospores. 12. Protocols, test and unknown strain microscopic examination.
Literature
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
Assessment methods (in Czech)
Podmínkou zápočtu je účast na cvičení, vypracování protokolů, úspěšné zpracování zápočtového preparátu a zápočtového testu.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/mikrob/obecna.html
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.