Bi5710c Microbiology - practice

Faculty of Science
Autumn 2015
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jana Čmielová, Ph.D. (seminar tutor)
Mgr. Gabriela Rotková, Ph.D. (seminar tutor)
Mgr. Tomáš Kastl (assistant)
Mgr. Veronika Vrbovská, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Miroslav Němec, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Gabriela Rotková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi5710c/01: Wed 8:00–9:50 E25/111, J. Čmielová, T. Kastl
Bi5710c/02: Thu 8:00–9:50 E25/111, J. Čmielová, V. Vrbovská
Bi5710c/03: Tue 13:00–14:50 E25/111, G. Rotková
Bi5710c/04: Tue 15:00–16:50 E25/111, Tue 17:00–18:50 E25/111, J. Čmielová
Prerequisites (in Czech)
NOW( Bi5710 Microbiology )
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the practical course of general microbiology students should be able to perform basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples).
Syllabus
  • 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition) 5. Determination of number of bacteria by cultivation method 6. Bacteria in water - basic microbiological analysis of water 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - use of biochemical tests and standardized Microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. Inoculation of microscopic fungi. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negative - staining, capsule - staining. Microscopic examination of endospores. 12. Protocols, written test and identification of unknown bacterial strain by Gram staining.
Literature
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
Teaching methods
Topic of each practical is at first explained and demonstrated to students. Then students are solving the practical task by themselves. The outcome of each practical is written report.
Assessment methods
Active participation on practicals, submission of reports, written test and identification of an unknown bacterial strain by Gram staining.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/mikrob/obecna.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2015, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2015/Bi5710c