PřF:Bi7430c Molecular biotechnology - pr. - Course Information
Bi7430c Molecular biotechnology - practical course
Faculty of ScienceAutumn 2007 - for the purpose of the accreditation
- Extent and Intensity
- 0/4/0. 4 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Alena Španová, CSc. (seminar tutor)
doc. Ing. Bohuslav Rittich, CSc. (seminar tutor)
RNDr. Marie Vojtíšková, CSc. (seminar tutor), doc. RNDr. Alena Španová, CSc. (deputy) - Guaranteed by
- doc. RNDr. Alena Španová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Španová, CSc. - Prerequisites (in Czech)
- NOW( Bi7430 Molecular biotechnology )
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12 - fields of study / plans the course is directly associated with
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Safety of work in and molecular biotechnical laboratory. GMO. Cultivation of bacterial E. coli JM109(pUC19)cells. Microisolation of plasmid DNA pUC19.Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. Isolation of recombinant DNA pUC19::dim1. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. Plating and selection of electrotransformants. Evaluation of electrotransformation. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. Dot blot. Southern blot. DNA/DNA hybridization at high stringent conditions. Immunological detection of hybridization products.
- Syllabus
- 1. Safety of work in and molecular biotechnical laboratory. GMO. 2. Cultivation of bacterial E. coli JM109(pUC19)cells. 3. Microisolation of plasmid DNA pUC19. 4. Cultivation of bacterial E. coli JM109(pUC19::dim1)cells. 5. Isolation of recombinant DNA pUC19::dim1. 6. Linearisation of plasmid DNA with restrictase EcoRI. Gel electrophoresis of DNA and estimation of restriction fragment lenght. 7. Transfer of recombinant and nonrecombinant plasmid DNAs into bacterial cells E. coli JM109 using electrotransformation. 8. Plating and selection of electrotransformants carrying recombinant and nonrecombinant plasmid DNA. Evaluation of electrotransformation. 9. Preparation of DNA probe from plasmid DNA - neradioactive labelling with digoxigenine. 10. Dot blot. 11. Southern blot. 12. DNA/DNA hybridization at high stringent conditions. 13. Immunological detection of hybridization products. 14. Evaluation of hybridization.Test. 15. Evaluation of protocols and test.
- Literature
- G. C. Saunders, H. C. Parkers. Analytical Molecular Biology. RSC. Cambridge 1999.
- F. Sambrook, R.W. Russell Molecular Cloning. A Laboratory Manual. 3rd ed. Cold Spring Harbor Laboratory Press. 2001.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.
- Enrolment Statistics (Autumn 2007 - for the purpose of the accreditation, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2007-forthepurposeoftheaccreditation/Bi7430c