Bi7430c Molecular biotechnology - practical course

Faculty of Science
autumn 2017
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (lecturer)
prof. RNDr. Jaroslav Turánek, DSc. (seminar tutor)
Mgr. Lukáš Chrást (seminar tutor)
Mgr. Veronika Lišková, Ph.D. (seminar tutor)
Mgr. Veronika Štěpánková, Ph.D. (seminar tutor)
Mgr. Tomáš Buryška, Ph.D. (seminar tutor)
Mgr. Šárka Nevolová, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable of Seminar Groups
Bi7430c/01: Mon 18. 9. to Fri 15. 12. Wed 10:00–11:50 Kontaktujte učitele, L. Chrást, Z. Prokop, V. Štěpánková
Bi7430c/02: Mon 18. 9. to Fri 15. 12. Wed 14:00–15:50 Kontaktujte učitele, L. Chrást, Z. Prokop, V. Štěpánková
Prerequisites
NOW( Bi7430 Molecular biotechnology )
Parallel attendance of Molecular Biotechnology lecture (Bi7430). The course of Molecular Biology (Bi4020) and Basics of molecular biology (Bi4010) completed.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 0/20, only registered: 0/20, only registered with preference (fields directly associated with the programme): 0/20
fields of study / plans the course is directly associated with
Course objectives
The practice provides an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts of the field and exercise stylistic skills of students in a writing an essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.
Learning outcomes
The practice provides to the students an interesting combination of practical problems solved under the guidance of experts from academia (MU and VRI) and business environment (a biotechnology company). In practical exercises the student will become trained in modern methods:

1. Design of recombinant systems

2. Fermentation of recombinant microorganisms

3. Preparation of enzymatic biosensor

4. Preparation and testing of microfluidic chip

5. Biodegradation of environmental pollutant by recombinant bacterium

6. Biocatalytic preparation of pharmaceutical precursor

7. Preparation and transformation of liposomes

8. Analysis of liposomes

At the end of the course, students will be familiar with a variety of techniques of molecular biotechnology, such as design of primers, DNA cleavage by restriction enzymes, transformation of bacterial cells, methods of fermentation culturing E. coli, application recombinant microorganisms and isolated enzymes in biosensing and remediation of hazardous substances or synthesis of pharmaceutically pure chemicals. The course will enable students to deepen their theoretical knowledge acquired in lectures Molecular Biotechnology. Students will become familiar with the currently used methods and the principle and operation of modern laboratory equipment. The course also aims to strengthen communication skills of students in a discussion with experts in the field and exercise stylistic skills of students in writing essays and protocols. The learning materials in English support the ability to work with scientific texts, including strengthening the knowledge of the English terminology used in this field.
Syllabus
  • 1. Design of recombinant systems
  • software tool Clone Manager, design of primers, restriction cleavage, cloning, transformation
  • 2. Preparation and testing of microfluidic chip
  • fabrication of PDMS chip, preparation and microscopy of microdroplets, microscopy of microdroplets with cells
  • 2. Fermentation of recombinant microorganisms
  • fermentation of E. coli biomass and expression of recombinant protein, technology EnBase
  • 3. Preparation of enzymatic biosensor
  • co-immobilization of recombinant enzyme and fluorescence probe to optical transducer, detection of selected analyte
  • 5. Biodegradation of environmental pollutant by recombinant bacterium
  • engineered bacterium, synthetic metabolic pathway, biodegradation of environmental pollutant, analysis of metabolites
  • 6. Biocatalytic preparation of pharmaceutical precursor
  • biocatalytic synthesis of pharmaceutical precursor by using recombinant enzyme
  • 7. Preparation and transformation of liposoms
  • preparation of liposomes, transformation, high-pressure extrusion, preparation of SUV (small unilamellar vesicels), DLS (dynamic light scattering) analysis, ZetaSizer
  • 8. Analysis of liposoms
  • single particle tracking analysis by using Nanosight 500, transmission electron microscopy
Literature
  • An introduction to molecular biotechnology : fundamentals, methods, and applications. Edited by Michael Wink. 2nd, update ed. Weinheim: Wiley-Blackwell, 2011, xxxiii, 60. ISBN 9783527326372. info
  • GLICK, Bernard R., Jack J. PASTERNAK and Cheryl L. PATTEN. Molecular biotechnology : principles and applications of recombinant DNA. 4th ed. Washington, D.C.: ASM Press, 2010, xvi, 1000. ISBN 9781555814984. info
Teaching methods
In the laboratory, each student works independently or in pairs under the guidance of the teacher. Each student works with own sample.

ORGANISATION OF PRACTICE
1. Theoretical introduction (presentation given by lecturer)
2. Introduction to practice and assignment control (homework)
3. Experimental work in the laboratory
Assessment methods
The results of experiments are evaluated by each student in separate essays and protocols based on the individual practice. Students will obtain positive assessment for active participation in the practice and preparation of protocols or essays from each exercise.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Přednost mají studenti specializace mikrobiologie.
Teacher's information
https://loschmidt.chemi.muni.cz/peg/courses/
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (autumn 2017, recent)
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