IB023 Binding and Kinetics for Experimental Biologists

Faculty of Science
Autumn 2010
Extent and Intensity
2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Petr Kuzmic, Ph.D. (lecturer), doc. Mgr. Ctirad Hofr, Ph.D. (deputy)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Course Enrolment Limitations
The course is offered to students of any study field.
The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30, only registered with preference (fields directly associated with the programme): 0/30
Syllabus
  • LECTURE TOPICS 1. Principles of data analysis: Matching models to experimental data 2. Confidence intervals for rate and equilibrium constants 3. Time course of biochemical and biophysical processes: Mathematical models 4. Experimental methods for the determination of rate constants 5. Biochemical and biophysical equilibria: Mathematical models 6. Analysis of equilibrium binding data 7. Enzyme catalysis and inhibition of enzymes 8. Choosing the appropriate molecular mechanism based on available data Notes: • All lectures will include concrete examples from published literature EXERCISES 1. Determination of a equilibrium binding constant for a protein-protein interaction 2. Determination of rate constants for protein-DNA interaction 3. Determination of stoichiometry protein-DNA interaction 4. Selecting an appropriate mechanism for enzyme inhibition Notes: • Practical sessions will be held in the computer laboratory using the software DynaFit • All exercises will include actual data published in the literature • Students are encouraged to bring their own additional data
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught only once.
The course is taught in blocks.
Note related to how often the course is taught: Výuka probíhá blokově 22.-26.11.2010.
General note: http://www.biokin.com/dynafit/index.html.
Information on the extent and intensity of the course: 1D.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2011 - acreditation.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2010/IB023