Bi7665 Cell and Tissue Cultures

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
Mgr. Jiří Pacherník, Ph.D. (lecturer)
Guaranteed by
Mgr. Jiří Pacherník, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Jiří Pacherník, Ph.D.
Prerequisites (in Czech)
Bi2080 Histology and organology
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the course the students are able to: Understand and explain principles of basic tissue culture techniques; Employ TC techniques in their own research; Routinely culture common cell lines; Characterize parameters of cell proliferation, differentiation, and apoptosis.
Syllabus
  • 1. Introduction to tissue culture; 2. basic pre-requisites for tissue culture techniques; 3. invertebrates and poikilotermic animals; 4.homeotermic animals; 5. germ and stem cells; 6. embryonic stem and embryonal carcinoma cells; 7. differentiation of embryonic stem and embryonal carcinoma cells; 8. primo-culture; 9. adherent tumor cell lines; 10. suspension tumor cell lines; 11. tissue ingineering and cell therapy; 12. culture of organs. Recommended literature Lesko, J. a kol.: Práce s tkanivovými kulturami, Bratislava, Vydavatelstvo slovenskej akademie ved 1975, s. 212. Alberts, B. a kol.: Základy buněčné biologie. Úvod do molekulární biologie buňky. Ústí nad Labem, Espero Publishing 1998, 630 s. Alberts, B. a kol.: Molekular biology of the cell. New York & London, Garland Publishing, Inc. 1994, 1294 s. Spector, D. L. a kol.: Cells. A laboratory manual. Volumes 1, 2, 3. New York, Cold Spring Harbor Laboratory Press 1998, 3 197 s. Cellis, J. E. a kol.: Cell Biology. A laboratory handbook. San Diego, London, Academic Press Inc. 1994, 1714 s. Sambrook, J. a kol.: Molecular Cloning. A laboratory manual. New York, Cold Spring Harbor Laboratory Press 1989, 1832 s. Ausubel, F. a kol.: Short Protocols in Molecular Biology. USA, Published by John Wiley & Sons Inc. 1995, 728 s.
Literature
  • Basic methods in microscopy : protocols and concepts from cells : a laboratory manual. Edited by David L. Spector - Robert D. Goldman. 1st ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 2006, ix, 382. ISBN 0879697512. info
Teaching methods
lectures
Assessment methods
Written test.
Language of instruction
Czech
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.