PřF:Bi7311 Mol. biol. of procar.-practice - Course Information
Bi7311 Molecular Biology of Procaryotes - Laboratory Course
Faculty of ScienceAutumn 2014
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Marian Varga, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Timetable of Seminar Groups
- Bi7311/01: Tue 13:00–13:50 E25/209, Tue 13:00–16:50 E25/119, R. Pantůček, V. Růžičková
Bi7311/02: Wed 8:00–8:50 E25/209, Wed 8:00–11:50 E25/119, R. Pantůček, V. Růžičková - Prerequisites
- ( B2050 General microbiology || Bi2050c General microbiology pr. || B4070 General mikrobiology || B4090 General microbiology || Bi4090c General microbiology - pr. ) && NOW( Bi7120 Molecular biology of prokaryotes )
Basic knowledge from molecular biology of the Prokaryotes. Basic techniques of microbiology. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 32 student(s).
Current registration and enrolment status: enrolled: 0/32, only registered: 0/32 - fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, N-EXB)
- Course objectives
- Practical course focused on molecular methods for analysis of procaryotic genome and basic methods of bacterial genetics. At the end of this course, students should be able to design and perform basic laboratory experiments focused on genetic studies of Escherichia coli and selected species of Gram-positive cocci.
- Syllabus
- 1. Pulsed-field gel electrophoresis of bacterial genomic DNA (preparation of samples, digestion with restriction endonuclease SmaI, PFGE).
- 2. Isolation of spontaneous mutants of Staphylococcus aureus resistant to antibiotics on gradient agar plates.
- 3. Fluctuation test and estimation of mutation rate.
- 4. Conjugation of Escherichia coli.
- 5. Isolation of auxotrophic mutants of Staphylococcus aureus. Replica plating.
- 6. Typing techniques used for epidemiological, and phylogenetic purposes, AP-PCR, RFLP.
- Literature
- MILLER, Jeffrey H. A Short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria. New York: Cold Spring Harbor Laboratory Press, 1992, 456 s. ISBN 0879693495. info
- ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
- Teaching methods
- The course is taught in research microbiology and molecular biology laboratories. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
- Assessment methods
- The course is completed after work out an elaborate and pass a writen test.
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
- Enrolment Statistics (Autumn 2014, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2014/Bi7311