PřF:Bi9393c Anal.cytometry - Pract.course - Course Information

Bi9393c Analytical cytometry - practical course

Extent and Intensity

0/1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Stanislav Drápela, Ph.D. (seminar tutor), Mgr. Radek Fedr (deputy)
Mgr. Markéta Hrušková (seminar tutor)
Ing. Michaela Chorvátová (seminar tutor)
Mgr. Barbora Kvokačková, Ph.D. (seminar tutor)
Mgr. Ondřej Vacek (seminar tutor)

Guaranteed by

Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16, only registered with preference (fields directly associated with the programme): 0/16

fields of study / plans the course is directly associated with

• Physiology (programme PřF, N-EBZ)
• Immunology (programme PřF, N-EBZ)
• Special Biology (programme PřF, N-EXB)
• Special Biology (programme PřF, N-EXB, specialization Experimentální biologie živočichů a imunologie)
• Developmental Biology (programme PřF, N-EBZ)

Course objectives

Practical training in basic methods of flow cytometry and high speed sorting.

Learning outcomes

The student will learn basic methods of cell analysis using flow cytometry including sample preparation acquisition and data analysis.

Syllabus

• Basic training in operation of routine flow cytometer FACSCalibur. Setup, calibration, quality control. Basic training in operation of high speed sorter. Setup, calibration, quality control. Cell cycle analysis. Immunophenotype analysis. GFP expression analysis and sorting. Data analysis - software FlowJo.

Literature

• Current Protocols in Cytometry, Print ISSN: 1934-9297 Online ISSN: 1934-9300

Teaching methods

practical training

Assessment methods

Evaluation of protocols.

Language of instruction

Czech

Follow-Up Courses

• Bi9393 Analytical cytometry

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!

Listed among pre-requisites of other courses

• Bi9393cen Analytical Cytometry – Practical Course
  (!Bi9393c) && !NOWANY(Bi9393c) && NOW(Bi9393en)  

• Enrolment Statistics (autumn 2021, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2021/Bi9393c