C7060 Trace Analysis

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Josef Komárek, DrSc. (lecturer)
doc. RNDr. Pavel Coufalík, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Josef Komárek, DrSc.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Wed 12:00–13:50 C14/207
Prerequisites
Knowledge of Analytical chemistry on the level of fundamental courses.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objective of the course is to obtain an overview and deeper knowledge in the field of trace analysis from clean laboratory, treatment of samples and flow analysis to preconcentration of trace elements. At the end of the course, students should be able to: - understand the problems of trace analysis - choose system of work in laboratory for trace analysis - characterize parameters important for environment of clean laboratory - compare capacities for methods for preconcentration of trace elements and separation of matrix - assess the advantages of flow analysis.
Learning outcomes
The student is able to: - utilize knowledge of requirements for environment of clean laboratory at work in this laboratory - use methods for preconcentration of trace elements by ultratrace analysis - assess the advantages of flow analysis.
Syllabus
  • 1. Special problems in work with small sample quantity and with extremely low concentration. Microgravimetry, microtitrations.
  • 2. Instrumental microanalysis, ultramicroanalysis.
  • 3. Importance of trace analysis for practice. Laboratory for trace analysis, its equipment and rules for users.
  • 4. Chemical vessels. Reagents, their purification and storing. Purity of chemicals and water.
  • 5. The sample, its homogeneity and storing. Contamination and analyte loses in the chemical operations.
  • 6. Decomposition of inorganic materials, pressure systems, bomb with teflon and quartz vessels.
  • 7. Decomposition of biological materials, wet ashing, oxidising mineral acids at normal pressure,high pressure digestion, microwave oven, Fentons reagent. Photolytic decomposition.
  • 8. High temperature dry ashing, muffle furnace, radio frequency plasma.
  • 9. Separation of trace and matrix elements. Volatilization.
  • 10. Selective dissolution, liquid-liquid extraction, sorption, ion exchange.
  • 11. Flow injection analysis, zone dispersion, FIA systems. Preconcentration of elements on chelating sorbents. Flow injection extraction system, segmentor, phase separator.
  • 12. Freezing and zone melting. Precipitation of trace elements, carrier precipitation and flotation.
  • 13. Electrochemical deposition, electrodeposition on solid electrodes and mercury cathodes, flow and static arrangement, Coupling of deposition with AAS. Blank and its importance in trace analysis.
  • 14. Local analysis of the surface, LA-ICP-OES.
  • 15. Trace analysis of organic compounds.
Literature
    recommended literature
  • Trace metal analysis and speciation. Edited by I. S. Krull. Amsterdam: Elsevier, 1991, 302 s. ISBN 0-444-88209-X. info
  • MIZUIKE, Atsushi. Metody koncentrirovanija mikroelementov v neoorganičeskom analize. Moskva: Chimija, 1986, 151 s. info
  • BEYERMANN, Klaus. Organic Trace Analysis. Chichester: Ellis Horwood Limited, 1984, 365 s. ISBN 0-85312-638-0. info
  • Dulski T.R.: Trace elemental analysis of metals. Methods and Techniques. 1999.
  • Coufalík P.: Stopová analýza kovů, 2019.
Teaching methods
Education is performed as lectures with PowerPoint presentation. Understanding of solution of trace analysis problems, preconcentration methods, flow analysis, data evaluation and the use in practical analysis is emphasized.
Assessment methods
Final assessment (at the end of semester) is by oral examination in the attendance or distance form. The exam consists in six questions, which require description and explanation of asked topic.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (autumn 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2021/C7060