Bi5710 Microbiology

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Monika Vítězová, Ph.D. (lecturer)
Mgr. Petr Králík, Ph.D. (lecturer), doc. Mgr. Monika Vítězová, Ph.D. (deputy)
Guaranteed by
doc. Mgr. Monika Vítězová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Ing. Jiřina Kučerová, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 15:00–16:50 B11/306
Prerequisites
! Bi4090 General microbiology && ! Bi2060 Basic microbiology && !NOWANY( Bi4090 General microbiology , Bi2060 Basic microbiology )
Examination from organic chemistry and biochemistry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
During the course the student will get acquainted with the basic concepts and principles of general and applied microbiology, including virology. They become familiar with the structure of prokaryotic and eukaryotic cells. Students will be informed about the principle of growth and multiplication of microorganisms and the influence of physical and chemical factors on microbial cells. Another aim of the subject will be an explanation of differences in the metabolism of photosynthetic, chemoautotrophic and heterotrophic microorganisms. Emphasis will be placed on clarifying the importance of microorganisms as pathogens and, on the contrary, their importance in the industry and in the environment. Course graduates will also learn about the most important eukaryotic microorganisms and their representatives.
Learning outcomes
Course graduates will be able to: - Understand and correctly apply the basic concepts and principles of general and applied microbiology, including virology;
- to distinguish and compare prokaryotes and eukaryotes, to compare archaea and bacteria;
- to list the properties and to explain the functions of the structures present in the bacterial cell, the archaeal cell and the cell of eukaryotic microorganisms;
- to distinguish photosynthetic, chemoatotrophic and heterotrophic microorganisms according to how they acquire energy;
- to demonstrate the importance of bacteria as pathogens and to describe the importance of selected microorganisms in industry and in the environment;
Syllabus
  • 1. Introduction to microbiology - recommended literature, assessment methods and criteria, history of microbiology. 2. Bacteria - cell structure and function, bacterial motility and endospores. 3. Microbial growth - growth curve, continuous cultivation of bacteria, influences of environmental factors on the growth. 4. Bacterial nutrition and metabolism - requirements for carbon, nitrogen, sulphur, phosphorus, growth factors, uptake of nutrients, introduction to metabolism. 5. Bacterial catabolism - chemoorganotrophic processes - aerobic and anaerobic respiration,fermentation, catabolism of carbohydrates, lipids and proteins, chemolithotrophy and phototrophy. Anabolism - principles, CO2 fixation, synthesis of sugars and polysaccharides, lipids, amino acids, purines, pyrimidines and nucleotides. Regulation of metabolism. 6. Bacterial genetics - chromosome, plasmids, gene expression, replication, transcription, translation, mechanisms of genetic variation. 7. Bacterial diseases, microbiology of food, applied environmental microbiology. 8. Cyanobacteria - cell structure, metabolism, ecology, industrial applications. 9. Archaea - cell structure and function, extremely halophilic and extremely thermophilic archaea, methanogenic archaea. 10. Yeasts - cell structure, metabolism, reproduction,yeasts as pathogens, industrial applications. 11. Fungi - fungal structure and function, reproduction and life cycles, common members of this group. 12. Viruses and other acellular agents - structure, viral multiplication, types of viral infections, cultivation of viruses, prions.
Literature
    required literature
  • NĚMEC, Miroslav and Dagmar MATOULKOVÁ. Základy obecné mikrobiologie (Introduction to General Microbiology). 1. vyd. Brno: Masarykova univerzita, 2015, 256 pp. ISBN 978-80-210-7923-6. info
    recommended literature
  • MADIGAN, Michael T. Brock biology of microorganisms. 12th ed. San Francisco, Calif.: Pearson/Benjamin Cummings, 2009, xxviii, 10. ISBN 9780132324601. info
  • NĚMEC, Miroslav and Dana HORÁKOVÁ. Základy mikrobiologie pro učitelské studium. 2. vyd. Brno: Masarykova univerzita v Brně, 1993, 233 s. ISBN 8021008172. info
  • KAPRÁLEK, František. Základy bakteriologie. Praha: Karolinum, 2000, 241 s. ISBN 8071848115. info
  • ŠILHÁNKOVÁ, Ludmila. Mikrobiologie pro potravináře a biotechnology. Vyd. 3., opr. a dopl., v Aca. Praha: Academia, 2002, 363 s. ISBN 8020010246. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1st ed. Brno: Neptun, 2001, 247 pp. ISBN 80-902896-2-2. info
  • WILLEY, Joanne M., Linda SHERWOOD and Christopher J. WOOLVERTON. Prescott's principles of microbiology. Edited by Lansing M. Prescott. 1st ed. Boston: McGraw-Hill Higher Education, 2009, xiv, 847. ISBN 9780071283670. info
Teaching methods
Lecture - Power Point presentation
Assessment methods
Final assessment is based on written examination. Final exam will be written multiple choice test consisting of 30 questions evaluated by 30 points. 17 points is needed to pass the final exam.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: písemný test prezenčně
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
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