Bi7270c Plant Embryology - Practice

Faculty of Science
Autumn 2022
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Hana Cempírková, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Hana Cempírková, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Naděžda Bílá
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 16:00–18:50 C13/332
Prerequisites (in Czech)
NOW( Bi7270 Plant Embryology )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 5 student(s).
Current registration and enrolment status: enrolled: 0/5, only registered: 0/5, only registered with preference (fields directly associated with the programme): 0/5
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to:
describe the development of angiosperm reproductive organs;
understand the processes of pollen-pistil interactions;
explain the process of double fertilization in angiosperms and to interpret seed development. Regulation of above mentioned processes will be illustrated on selected plant models.
Learning outcomes
At the end of this course, students should be able to:
describe the development of angiosperm reproductive organs;
understand the processes of pollen-pistil interactions;
explain the process of double fertilization in angiosperms and to interpret seed development. Regulation of above mentioned processes will be illustrated on selected plant models.
Syllabus
  • Seed as a start of a new life cycle: angiosperm seed structure (testa, endosperm, aleuron layer, embryo: embryonal axis, cotyledones, hypocotyl), seed quiescence and dormancy, seed germination, seedling structure.
  • Mitosis - nuclear division: Cell cycle, cytoskeleton formation during cell cycle, polarity, asymmetric divisions, squash methods, pretreatments for karyology, cell cycle synchronization.
  • Morphology of the vegetative stem apical meristem (structure, stem cells, leaf primordia and leaf meristems, leaf differentiation); root apical meristem (quiescent centre, root cap, columela).
  • Megagametogenesis, gynaeceum, pistil, angiosperm ovule structure, clearing methods of plant ovules, observation of cleared ovules by means of Nomarski DIC.
  • Microsporogenesis and microgametogenesis, androeceum, stamen: anther and filamentum, structure of the anther wall, tetrades, types of pollen grains, differential staining of pollen grains, determination of pollen viability.
  • Pollen grain germination in vitro, phase contrast microscopy.
  • Pollen - pistil interaction: pollen tube growth, callose plugs, decolorised aniline blue staining, fluorescence microscopy in UV light.
  • Cryostat sections in embryology - fixation, cryoprotection, cryo-sectioning (cryostat), enzyme histochemistry, histochemical reactions of hydrolytic enzymes.
  • Histological techniques in plant embryology I. - fixation of isolated organs.
  • Histological techniques in plant embryology II. Dehydration of samples, paraffin and resin embedding, microtome sectioning, adhesion of sections, de-waxing of sections.
  • Histological techniques in plant embryology III. Successive double staining of sections, dehydration and Eukitt mounting.
  • Documentation of prepared microscopic preparations - drawings, measurements, micrographs, automatic analysis of images.
Literature
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
  • FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
  • KIERNAN, J. A. Histological & histochemical methods : theory and practice. 3rd ed. London: Arnold, 2003, x, 502. ISBN 0750649364. info
Teaching methods
Laboratory course.
Assessment methods
Group projects, elaboration of protocols.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
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