C4221 Biochemical Lab Technique

Faculty of Science
Autumn 2022
Extent and Intensity
0/0/4. 4 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C7777 Handling chemical substances && C4222 Basic calculations
Theoretical knowledge and practical skills of the high school chemistry level
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 18 student(s).
Current registration and enrolment status: enrolled: 3/18, only registered: 0/18
fields of study / plans the course is directly associated with
there are 9 fields of study the course is directly associated with, display
Course objectives
The aim of the course is to obtain basic laboratory skills (safety and fire rules, basic rules for safe chemical laboratory practice and basic biochemistry operations)
Learning outcomes
Basic laboratory operations as well as determination of physicochemical constants of compounds are exercised in this course. Students will learn in this course safety and fire rules and basic rules for safe chemical laboratory practice and perform basic laboratory operations and manipulation with chemical laboratory equipment and simple instruments
Syllabus
  • 1. laboratory safety rules 2. glassblower work 3. liquid volume measurements, pipetting 4. weighing, statistics 5. thin layer chromatography 6. spectrophotometry 7. pH measurement 8. separation methods 9. vacuum distillation 10. steam distillation 11. titration 12. microscopy
Literature
  • ČECH, Svatopluk. Aleš Mareček, Antonín Růžička: Laboratorní technika pro nechemické obory. Masarykova univerzita ve spolupráci s Chemickou fakultou VUT, Brno, s.44, ISBN 80-210-1653-1. (Aleš Mareček, Antonín Růžička: Laboratory technique for students of non-chemical specializations.). Univerzitní noviny. Brno, 1998, vol. 5, No 10, p. 58. ISSN 1211-6866. info
  • KEIL, Bořivoj. Laboratorní technika biochemie. 1. vyd. Praha: Nakladatelství Československé akademie věd, 1959, 872 s. info
Teaching methods
laboratory courses
Assessment methods
The laboratory course runs on a weekly basis. Each student is tested at the beginning of the class for the theoretical knowledge of the particular experiment by a short written test or by questions.
Language of instruction
Czech
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2011, Spring 2012, Autumn 2011 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Autumn 2016, Spring 2017, autumn 2017, spring 2018, Autumn 2018, Spring 2019, Autumn 2019, Spring 2020, Autumn 2020, Spring 2021, autumn 2021, Spring 2022, Spring 2023, Autumn 2023, Spring 2024, Autumn 2024, Spring 2025.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/C4221