C9320 Methods of biochemical research

Faculty of Science
Autumn 2022
Extent and Intensity
0/0/6. 6 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. Mgr. Karel Kubíček, PhD. (seminar tutor)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. et Mgr. Lenka Kohútová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)
Mgr. Josef Houser, Ph.D. (seminar tutor)
Mgr. Monika Kubíčková, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Zdeněk Farka, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C9320/01: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/02: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/03: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/04: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
C9320/05: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, J. Houser, L. Kohútová, K. Kubíček, M. Kubíčková, J. Lochman, M. Mandl, V. Sedláček, P. Skládal, O. Šedo
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 12 fields of study the course is directly associated with, display
Course objectives
In the laboratory exercise, students will be acquainted with practical features and basic applications of the methods listed in syllabus.
Learning outcomes
Student will be able:
- to understand basic principles of representative instrumental methods used in biochemistry;
- to use various methods for experimental work in biochemistry and related areas.
Syllabus
  • 1. SDS-PAGE
  • 2. HPLC
  • 3. Biosensors
  • 4. Spectrophotometry
  • 5. Transfer of oxygen
  • 6. RT-qPCR
  • 7. Crystallography
  • 8. MALDI-MS
  • 9. Ultracentrifugation
  • 10. NMR
  • 11. CZE
  • 12. Immunoassays
Literature
    recommended literature
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986, 199 s. info
Teaching methods
Individually supervised laboratory course.
Assessment methods
Twelve exercises have to be completed according to the schedule.
Credit completion on the base of participation and protocol processing.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: předmět není možné zapsat v bakalářském programu; v pětiletém magisterském programu je možné předmět zapsat nejdříve ve 4. ročníku
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/C9320