C9320 Methods of biochemical research

Faculty of Science
Autumn 2024
Extent and Intensity
0/0/6. 6 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
prof. RNDr. Petr Skládal, CSc. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
prof. RNDr. Zdeněk Glatz, CSc. (seminar tutor)
doc. Mgr. Karel Kubíček, PhD. (seminar tutor)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Ondrej Šedo, Ph.D. (seminar tutor)
Mgr. Josef Houser, Ph.D. (seminar tutor)
Mgr. Jitka Holková (seminar tutor)
Mgr. Michal Sedlák (seminar tutor)
Guaranteed by
doc. Mgr. Zdeněk Farka, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C9320/01: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, Z. Glatz, J. Holková, J. Houser, K. Kubíček, J. Lochman, M. Mandl, V. Sedláček, M. Sedlák, P. Skládal, O. Šedo
C9320/02: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, Z. Glatz, J. Holková, J. Houser, K. Kubíček, J. Lochman, M. Mandl, V. Sedláček, M. Sedlák, P. Skládal, O. Šedo
C9320/03: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, Z. Glatz, J. Holková, J. Houser, K. Kubíček, J. Lochman, M. Mandl, V. Sedláček, M. Sedlák, P. Skládal, O. Šedo
C9320/04: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, Z. Glatz, J. Holková, J. Houser, K. Kubíček, J. Lochman, M. Mandl, V. Sedláček, M. Sedlák, P. Skládal, O. Šedo
C9320/05: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, Z. Glatz, J. Holková, J. Houser, K. Kubíček, J. Lochman, M. Mandl, V. Sedláček, M. Sedlák, P. Skládal, O. Šedo
C9320/06: Wed 7:00–12:50 Kontaktujte učitele, P. Bouchal, K. Dadáková, Z. Farka, Z. Glatz, J. Holková, J. Houser, K. Kubíček, J. Lochman, M. Mandl, V. Sedláček, M. Sedlák, P. Skládal, O. Šedo
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 12 fields of study the course is directly associated with, display
Course objectives
In the laboratory exercise, students will be acquainted with practical features and basic applications of the methods used in biochemical research. First, the instrumentation will be introduced, followed by the practical training of the students in its control, including sample prepration, measurement, and data analysis.
Learning outcomes
Student will be able:
- to understand basic principles of representative instrumental methods used in biochemistry;
- practically acquainted with the methods of biological sample preparation, control of moder laboratory instrumentation, and data analysis;
- to use various methods for experimental work in biochemistry and related areas.
Syllabus
  • 1. Biosensors
  • 2. Immunoassays
  • 3. NMR
  • 4. SDS-PAGE
  • 5. Spectrophotometry
  • 6. Transfer of oxygen
  • 7. RT-qPCR
  • 8. Crystallography
  • 9. MALDI-MS
  • 10. HPLC
  • 11. CE
  • 12. Calorimetry
Literature
  • HOLTZHAUER, Martin. Basic methods for the biochemical lab. 1st english ed. Berlin: Springer, 2006, xviii, 251. ISBN 3540327851. info
  • PINGOUD, Alfred. Biochemical methods : a concise guide for students and researchers. Weinheim: Wiley-VCH, 2002, xiii, 360. ISBN 3527302999. info
Teaching methods
Individually supervised laboratory course.
Assessment methods
Twelve exercises have to be completed according to the schedule.
Credit completion on the base of participation and protocol processing.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: předmět není možné zapsat v bakalářském programu; v pětiletém magisterském programu je možné předmět zapsat nejdříve ve 4. ročníku
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2024/C9320