CB080 Protein crystallography - practice

Faculty of Science
Autumn 2022
Extent and Intensity
0/1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jaromír Marek, Ph.D. (seminar tutor)
Mgr. Tomáš Klumpler, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Jaromír Marek, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Tue 13:00–13:50 C04/211
Prerequisites (in Czech)
NOW( CB070 Protein crystallography )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
CB080 is the exercise connected to the course CB070. The aim of the subject is to teach students to perform three basic steps of protein diffraction crystallography: to process synchrotron diffraction data (= data reduction), to solve the phase problem and to perform refinement of the model.
Learning outcomes
CB080 is the exercise connected to the course CB070. Student will be trained in processing of synchrotron diffraction data and work with PDB, and he/she will understand basic principles of work with CCP4 and a map of electron density too.
Syllabus
  • Sources and detectors of X-ray. Diffractometer, area detectors
  • Program XDS
  • System CCP4
  • Phase problem, molecular replacement
  • Programs for MR
  • R-factors, Least-square-method
  • Programs SHELXS and SHELXL, CNS and CCP4.
  • Refinement of protein structures, electron density maps
  • Program Coot
  • Crystallographic databases.
Literature
  • MAREK, Jaromír and Z. TRÁVNÍČEK. Monokrystalová rentgenová strukturní analýza (Single crystal X-ray structure analysis). první. Olomouc: Vydavatelství Univerzity Palackého, 2002, 169 pp. nedělí se na edice. ISBN 80-244-0551-2. info
  • GIACOVAZZO, C. Fundamentals of Crystallography. 1992. ISBN 0-19-855578-4. info
Teaching methods
Exercises for theoretical course CB 070. Home exercises at PC.
Assessment methods
Credit. Home exercises at PC.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/CB080