PřF:Bi9250c Special Immunol. Meth. - pr. - Course Information

Bi9250c Special Immunological Methods - practical course

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

doc. Mgr. Lukáš Kubala, Ph.D. (seminar tutor), doc. RNDr. Alena Žákovská, Ph.D. (deputy)
Mgr. Jiří Petráš (assistant)
doc. RNDr. Alena Žákovská, Ph.D. (seminar tutor)

Guaranteed by

doc. RNDr. Alena Žákovská, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Lukáš Kubala, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Bi5220 Immunology && Bi5220c Immunology - pr.
Subject is connected to the basic practice course in immunology that is why students are supposed to have finished the basic practice course in immunology. The course is to extended the theory and praxis of basic immunology subject in its methods.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 14 student(s).
Current registration and enrolment status: enrolled: 18/14, only registered: 0/14, only registered with preference (fields directly associated with the programme): 0/14

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;

Learning outcomes

At the end of the course students should be able to: understand and explain basic methods in immunology; use data about these methods in praxis; make reasoned decisions about the method ELISA; interpret its practical knowledge in labs; understand and explain the basic immunological techniques; work with information on important current immunological methods used in immunological labs and in research;

Syllabus

• The practice proceeds in the form of individual parts of methods that are solved: 1. putting of antigen on the surface of microtitration plates and their preparation for ELISA test
• 2. testing on the presence of antibodies against chosen pathogen in serum
• 3. Test for the detection of lymphocyte activity
• 4. Imunochromatographic tests - test for the detection of the presence of HIV, RSV antibodies in sera
• 5. Test of indirect agglutination. Presence of erytrhrocytes Ag in saliva
• 6. Immunocytology - invertebrate immunology, immune cells of insects

Literature

• Bartůňková J., Paulík M. a kol.:Vyšetřovací metody v imunologii
• Toman M., a kol. Veterinární imunologie, Grada Publishing 2000
• Hořejší V., Bartůňková J.: Základy imunologie, Triton Praha 1998
• Ferenčik M.: Imunochémia, 2. Vydání, Alfa Bratislava 1989

Teaching methods

laboratory exercising; using some of simple and difficult immunological methods.

Assessment methods

The students participation at lectures is mandatory. The final classification is in the form of written test at the end of term. The students are recommended to fill in written self-tests offered during the term.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!

Listed among pre-requisites of other courses

• Bi9250cen Special Methods of Immunology – Practical Course
  (!Bi9250c) && !NOWANY(Bi9250c) && NOW(Bi5220en)  

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