PřF:B8090 Gene engineering - Course Information

B8090 Gene engineering

Faculty of Science
Spring 2000

Extent and Intensity

3/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Jiří Doškař, CSc. (lecturer)

Guaranteed by

Prerequisites (in Czech)

B3060 Basic genetics && ( B4020 Molecular biology || B4030 Molecular biology || B6130 Molecular biology ) && ( B6390 Molecular Methods || B6400 Methods of molecular biology ) && B7120 Molecular biology of prokaryotes && NOW( B8313 Genetic engineering - practice )

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Syllabus

Gene engineering, its history and perspective. In vitro mutagenesis as a method for changing genes, random mutagenesis, primer extension methods, cassette mutagenesis, PCR methods of site-directed mutagenesis. Protein engineering. Optimizing the expression of cloned genes, factor affecting the gene expression in a new host. Cloning in Gram-positive bacteria. Eukaryotic vectors, selectable marker for eukaryotic cells. Transfer of recombinant DNA into eukaryotic organisms. Cloning in yeast. Gene transfer to plants. Cloning in animal cells. Transferring genes into animal oocytes, eggs, embryos and specific animal tissues. Introduction of foreign genes into mice. Transgenic organisms. Gene therapy. Application of recombinant DNA to biotechnology. The impact of recombinant DNA technology.

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

The course is also listed under the following terms Spring 2001, Spring 2002.