PřF:C6850 Chromatographic Methods II. - Course Information
C6850 Chromatographic Methods II.
Faculty of ScienceSpring 2002
- Extent and Intensity
- 2/0/0. 3 credit(s). Type of Completion: zk (examination).
- Teacher(s)
- doc. RNDr. Aleš Hrdlička, CSc. (lecturer)
RNDr. Miroslav Ciganek, Ph.D. (lecturer) - Guaranteed by
- doc. RNDr. Aleš Hrdlička, CSc.
Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Aleš Hrdlička, CSc. - Prerequisites
- Knowledge of fundamental chromatographic principles and parameters corresponding to the level of introductory part of C5910 course, Chromatographic methods I.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- there are 19 fields of study the course is directly associated with, display
- Course objectives
- The course is a continuation of C5910 Chromatographic Methods I. Principles, techniques, instrumentation and contemporary trends in the field of capillary electrophoresis (HPCE), capillary electrochromatography (CEC), and gas chromatography (HRGC) are discussed in details.
- Syllabus
- I. Capillary electrophoresis and electrochromatography. II.1 Ion progression in electrical field, fundamental equations, terms, and parameters. II.2 Principles of CE techniques and those of capilllary electrochromatography, applications. II.3 Instrumentation. Quantitation. II. Gas chromatography. II.1 Retention characteristics, Gas flow in porous media, van Deemter equation and Golay equation. Retention indexes. II.2 Gas-Liquid Chromatography. Columns, liquid phases and their characterization, supports. II.3 Adsorption gas chromatography. Features, comparison with GLC. Adsorbents, applications. II.4 Capillary columns, packed, WCOT, PLOT, SCOT. Quality evaluation. II.5 Mobile phase, comparison of gases. II.6 Instrumentation, sample injection, detection. GC/MS, GC/AED, and GC/FTIR hyphenation. Multidimensional GC. Parameter programming.
- Literature
- ROOD, D. A Practical Guide to the Care, Maintenance, and Troubleshooting of Capillary Gas Chromatographic Systems. Heidelberg: Huethig Buch Verlag, 1991. Chromatographic Methods. ISBN 3-7785-1898-4. info
- POOLE, C. F. and S. K. POOLE. Chromatography Today. 5th Impression. Amsterdam: Elsevier, 1997. ISBN 0-444-89161-7. info
- Assessment methods (in Czech)
- přednášky, ústní zkouška
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (Spring 2002, recent)
- Permalink: https://is.muni.cz/course/sci/spring2002/C6850