C4220 Biochemistry - laboratory course

Faculty of Science
Spring 2003
Extent and Intensity
0/0/7. 7 credit(s). Type of Completion: graded credit.
Teacher(s)
RNDr. Pavla Boublíková, CSc. (seminar tutor)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
Mgr. Michal Kuňák, Ph.D. (seminar tutor)
doc. Ing. Martin Mandl, CSc. (seminar tutor)
Jiří Mazoch (seminar tutor)
prof. RNDr. Vladimír Mikeš, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C4220/Bc1: No timetable has been entered into IS. P. Boublíková
C4220/Bc2: No timetable has been entered into IS. O. Janiczek
C4220/Ch1: No timetable has been entered into IS. P. Bouchal
C4220/Ch2: No timetable has been entered into IS. M. Kuňák
C4220/Ch3: No timetable has been entered into IS. M. Mandl
C4220/Ch4: No timetable has been entered into IS. J. Mazoch
Prerequisites
C3181 Biochemistry I
Basic knowledge of Biochemistry I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative reactions of carbohydrates, lipids, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of free enzymes as well as enzymes which are immobilized in cells.
Syllabus
  • Qualitative reactions of carbohydrates. Determination of glucose using a biosensor. Carbohydrate thin-layer chromatography.
  • Isolation and identification of lipids from the egg yolk.
  • Protein electrophoresis of the blood serum. Determination and chromatographic separation of leaf pigments. Gel permeation chromatography in separation of biomolecules.
  • Chemical reactions of amino acids and proteins. Determination of amino acids and peptides. Paper chromatography of amino acids. Protein isolation.
  • Isolation and properties of nucleic acids.
  • Heat dependence of enzyme reaction.
  • Protein determination.
  • pH effect on enzyme reaction. Determination of the rate of enzyme reaction.
  • Substrate specifity of enzymes. Determination of enzyme activity.
  • Enzyme kinetics. Enzyme inhibition.
  • Enzymatic determination of metabolites.
  • Electron transport chain of aerobic organisms.
  • Closing test.
Literature
  • MIKEŠ, Vladimír. Základní biochemické praktikum. 3. vyd. Brno: Masarykova univerzita, 1997, 113 s. ISBN 8021015306. info
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Assessment methods (in Czech)
První cvičení je věnováno poučení z bezpečnosti práce v biochemické laboratoři. Před každým dalším cvičením se písemným testem ověřuje příprava studenta na danou úlohu. Závěrečný test ověřuje znalosti z principů všech úloh.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
General note: Vyučují učitelé katedry biochemie.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2003, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2003/C4220