C4220 Biochemistry - laboratory course

Faculty of Science
Spring 2024
Extent and Intensity
0/0/7. 7 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. Lukáš Faltinek (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor)
Mgr. Alexandra Chomová (assistant)
Ing. Matěj Masař (assistant)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C4220/01: Mon 19. 2. to Sun 26. 5. Mon 8:00–14:50 C15/212, M. Pelcová
C4220/02: Mon 19. 2. to Sun 26. 5. Wed 8:00–14:50 C15/212, L. Faltinek, A. Chomová
C4220/03: Mon 19. 2. to Sun 26. 5. Thu 8:00–14:50 C15/212, L. Faltinek
C4220/04: Mon 19. 2. to Sun 26. 5. Fri 8:00–14:50 C15/212, K. Dadáková
C4220/05: Mon 19. 2. to Sun 26. 5. Tue 12:00–18:50 C15/212, J. Kučera, M. Masař
Prerequisites
C3181 Biochemistry I && ( C4221 Introductory Biochemistry Laboratory || C1100 Introductory Chemistry Lab )
Basic knowledge of Biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of metabolic pathways. Physiological processes and their biochemical nature. Basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes.
Learning outcomes
Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work
Syllabus
  • 01. Work safety rules. Organization of laboratory exercises. Preparation of dilute solution, pipetting, and photometry.
  • 02. Isolation, analysis, and determination of deoxyribonucleic acid (DNA).
  • 03. Qualitative and quantitative determination of amino acids.
  • 04. Quantitative determination of proteins.
  • 05. Qualitative and quantitative determination of carbohydrates.
  • 06. Lipids and their analysis.
  • 07. Sucrase - immobilization, substrate specificity, activity determination.
  • 08. pH optimum of enzyme reaction.
  • 09. Enzyme kinetics.
  • 10. Analytical use of enzymes.
  • 11. Biological electron transfer chains.
Literature
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Teaching methods
laboratory courses
Assessment methods
The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 65% of the questions.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Information on course enrolment limitations: V podzimním semestru se vypisuje pouze jedna skupina pro 12 studentů chemických oborů jako náhrada za JS
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.
  • Enrolment Statistics (Spring 2024, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2024/C4220