C4220 Biochemistry - laboratory course

Faculty of Science
Spring 2009
Extent and Intensity
0/0/7. 7 credit(s). Type of Completion: graded credit.
Teacher(s)
RNDr. Pavla Boublíková, CSc. (lecturer)
doc. Mgr. Pavel Bouchal, Ph.D. (seminar tutor)
doc. RNDr. Oldřich Janiczek, CSc. (seminar tutor)
prof. Mgr. Tomáš Kašparovský, Ph.D. (seminar tutor)
doc. Mgr. Jan Lochman, Ph.D. (seminar tutor)
doc. RNDr. Stanislav Pavelka, CSc. (seminar tutor)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable of Seminar Groups
C4220/01: Mon 13:00–18:50 C05/108, P. Boublíková
C4220/02: Tue 7:00–12:50 C05/108, P. Boublíková
C4220/03: Wed 7:00–12:50 C05/108, T. Kašparovský
C4220/04: Thu 7:00–12:50 C05/108, P. Boublíková
C4220/05: Tue 13:00–18:50 C05/108, S. Pavelka
C4220/06: Thu 13:00–18:50 C05/108, P. Bouchal
C4220/07: Wed 13:00–18:50 C05/108, P. Boublíková
Prerequisites
C3181 Biochemistry I
Basic knowledge of Biochemistry I
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
there are 21 fields of study the course is directly associated with, display
Course objectives
The objective of the laboratory course are studies of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes. Content of the first part includes qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part is oriented to enzyme kinetics and further studies on properties of enzymes.
Syllabus
  • Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes.Imobilization of enzymes.Respiratory chain chain of aerobic organisms.
Literature
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995, S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Assessment methods
credit course
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Information on course enrolment limitations: V podzimním semestru se vypisuje pouze jedna skupina pro 12 studentů chemických oborů jako náhrada za JS
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2009, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2009/C4220