C4050 Analytical Chemistry II

Faculty of Science
Spring 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Josef Havel, DrSc. (lecturer)
prof. RNDr. Přemysl Lubal, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Josef Havel, DrSc.
Chemistry Section – Faculty of Science
Prerequisites (in Czech)
C3100 Analytical Chemistry I
Předpokladem je absolvování základních přednášek především z Analytické chemie I (C3100), dále z Fyzikální chemie I (C3140) z Obecné chemie (C1020), Anorganické chemie I (C1061), Anorganické chemie II (C2062), Organické chemie I (C2021), Organické chemie II (C3022) a příslušných laboratorních cvičení z těchto předmětů (hlavně Analytická chemie - laboratorní cvičení (C3120)).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
A systematic treatment of the basic principles of analytical chemistry for students of chemistry - part II. The course includes electroanalytical, optical and separation methods.
Syllabus
  • 1. Introduction into subject instrumental analytical chemistry. Evaluation of results of analysis - statistics and GLP knowledge. Physical properties and analytical signal, relationship of analytical signal and concentration, calibration curves and their evaluation, standardisation for relative physical methods. Parameters of analytical method (detection limit, limit of determination, sensitivity, robustness, , precision, accuracy, etc.). Errors and their relationship to analytical method parameters. Statistical evaluation of analytical results - Gauss, Student and Dean-Dixon approach). Definition and parameters for precision and accuracy of analytical method. Rejection of outliers, circle test.
  • 2. Chosen electroanalytical methods. Potentiometric methods, basic definitions and terms. Indicator and reference electrodes. pH determination. Potentiometric indication of course of titration and equivalence point. Acid-base, redox, precipitation, complexometric titrations with potentiometric indication. Reductometric titrations with potentiometric indication. Potentiometric evaluation of equivalence point (Gran linearization of titration curve).
  • 3. Conductometric methods - basic definitions and terms. Direct conductometry, application for conductometric determination of equivalence point.
  • 4. Electrogravimetry, coulometry - basic definitions and terms. Polarisation curves, deposition current, faradaic current. Electrolysis under constant potential and constant current. Electrolytic separation of metals, determination of copper and silver. Calculations of potentials for deposition, coulometry under constant current. Coulometric titrations (absolute titration methods).
  • 5. Voltametry, polarography - basic definition and terms. Polarographic analysis (quantitative and qualitative analysis). Examples of applications and determinations. Amperometric, biamperometric and bipotentiometric titrations. Karl-Fischer determination of water.
  • 6. Chosen optical methods. Introduction - description of electromagnetic radiation, basic definition and terms, relationships (e.g. Bouger - Lambert-Beerův law), origin of absorption and emission of light. Division of optical analytical methods. It is necessary to know the light sources, dispersion elements, detectors, methodology.
  • 7. Molecular absorption spectroscopy (UV, VIS, IR).
  • 8. Atomic absorption and emission spectroscopy. Luminescent methods.
  • 9. NMR a EPR spectrometry. Molecular scattering spectroscopy (turbidimetry and nephelometry).
  • 10. Chosen separation methods. Solvent extraction - basic terms and definition. Extraction equilibria in two-phase system. Solvate extraction, ionic and nonionic substances. Analytical use of ion exchangers - basic terms and definition. Solid and liquid ion exchangers - characteristic, relationships and examples of the applications.
  • 11. Chromatography on thin layer of sorbent (thin layer chromatography, paper chromatgraphy) - principles and examples of applications. Gas analysis - classic (principle and examples of applications), gas chromatography (theory, basic description of instrumentation, qualitative and quantitative analysis, examples of analytical applications).
  • 12. HLPC - high performance chromatography (theory, basic description of instrumentation, qualitative and quantitative analysis, examples of analytical applications). Electromigration methods (zone electrophoresis, electrophoresis on carriers and izotachophoresis} - basic definitions and terms, kinds of methods, basic description of instrumentation, examples of analytical applications).
  • 13. Foundations of analysis of organic compounds. Qualitative and quantitative characteristic - general principles. Elemental analysis, analysis of functional groups - examples, determination of compound purity, methodology for structure determination of organic compounds. Determination of compounds in more complex mixtures.
Literature
  • Sommer L a kol., Základy analytické chemie II, VUTium Brno 2000.
  • Analytická příručka. Edited by Jaroslav Zýka. 4., upr. vyd. Praha: SNTL - Nakladatelství technické literatury, 1988, 831 s. info
  • SKOOG, Douglas A., James F. HOLLER and Timothy A. NIEMAN. Principles of instrumental analysis. 5th ed. Philadelphia: Saunders College Publishing, 1998, xv, 849 s. ISBN 0-03-002078-6. info
  • HARRIS, Daniel C. Quantitative chemical analysis. 4th ed. New York: W.H. Freeman, 1995, xix, 837 s. ISBN 0-7167-2508-8. info
  • Kellner R., Mermet J.-M., Otto M., Widmer H. M., Analytical Chemistry, Wiley 1998.
Assessment methods (in Czech)
2 hodinová přednáška
kombinovaná zkouška (písemná a ústní část)
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2004, recent)
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