PřF:C4700 Vybrané biochem.metody-cvič.

C4700 Vybrané biochemické metody - cvičení

Faculty of Science
Spring 2005

Extent and Intensity

0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).

Teacher(s)

doc. RNDr. Petr Zbořil, CSc. (seminar tutor)

Guaranteed by

Timetable

Fri 13:00–14:50 Cpm,02016

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

Course objectives

Important biochemical laboratory methods for biochemical analysis and diagnostics. Spectrophotometry, fluorometry, chromatography, electrophoresis.

Syllabus

Theoretical backgrounds. Principles, instrumentation and use of most important separation and other methods in biochemistry. Principles of work with biochemical material. Theory of chromatography and electromigration methods. Basic spectral methods. Practical training in absorption spectra measurement, photometric estimation of enzyme activities. Flurescent behaviour of typical fluorochromes, influence of environment. Protein separation using high performance liquid chromatography. Polyacrylamide gel electrophoresis of proteins.

Literature

KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987, 430 s. URL info

Assessment methods (in Czech)

Cvičení. Zápočet.

Language of instruction

Czech

Further Comments

The course is taught annually.