C4700 Special Biochemical Methods - Practice

Faculty of Science
Spring 2020
Extent and Intensity
0/2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Mgr. Matěj Pastucha, Ph.D. (seminar tutor)
doc. Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Mgr. Aleš Mádr, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to understand and explain important biochemical laboratory methods for biochemical analysis and diagnostics - spectrophotometry, fluorometry, chromatography, electrophoresis.
Learning outcomes
After finishing the course, student will be able to carry out special biochemical measurements using devices as fluorimeter, HPLC and SECM.
Syllabus
  • Theoretical backgrounds. Principles, instrumentation and use of the most important separation and other methods in biochemistry. Principles of work with biochemical material. Theory of chromatography and electromigration methods. Basic spectral methods. Practical training in absorption spectra measurement, photometric estimation of enzyme activities. Fluorescent behaviour of typical fluorochromes, influence of environment. Protein separation using high performance liquid chromatography. Polyacrylamide gel electrophoresis of proteins.
Literature
  • KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987, 430 s. URL info
Teaching methods
Laboratory excercise.
Assessment methods
Laboratory course. Obtaining of credit requires presence in courses and written protocols.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2020/C4700