Bi4090c General microbiology - practical course

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Monika Vítězová, Ph.D. (seminar tutor)
doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
Guaranteed by
RNDr. Ludmila Tvrzová, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 7 fields of study the course is directly associated with, display
Course objectives
The aim of the practical course of general microbiology are basic methods used in microbiological laboratory (methods of sterile inoculation and cultivation, slides preparation and microscopic examination of microorganisms, determination of the number of bacteria in different samples).
Syllabus
  • 1. Introduction, safety in microbiological laboratory 2. Media for cultivation and their preparation 3. Isolation, inoculation and cultivation of Microorganisms 4. Microscopic examination of bacteria (slides preparation, Gram - staining, microscopy and image acquisition 5. Determination of number of bacteria by cultivation method 6. Bacteria in water - basic microbiological water analysis 7. Determination of the concentration of antibiotics by microbiological methods. Determination of susceptibility of bacteria to antibiotics 8. Identification of bacteria - the use of biochemical tests and standardized Microsystems 9. Yeasts, test of vitality 10. Microorganisms in soil. Inoculation of microscopic fungi. 11. Isolation and microscopic examination of bacteria from soil (cellulolytic bacteria, Azotobacter sp., Clostridium sp.), negativ - staining, capsule - staining. Microscopic examination of endospores. 12. Protocols, test and unknown strain microscopic examination.
Literature
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
Assessment methods (in Czech)
Podmínkou zápočtu je účast na cvičení, vypracování protokolů, úspěšné zpracování zápočtového preparátu a zápočtového testu.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
Teacher's information
http://www.sci.muni.cz/mikrob/obecna.html
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.