Bi6150c Mycorrhizal symbioses - practice

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Milan Baláž, Ph.D. (seminar tutor)
Guaranteed by
RNDr. Milan Baláž, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová
Prerequisites (in Czech)
NOW( Bi6150 Mycorrhizal symbioses )
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the practical course is to familiarize the students with basic methods of current mycorrhizal research.
Syllabus
  • Mycorrhizal colonization of plant roots: review of commonly used methods, assessment of root colonization (chemical and microscopic methods), observation of typical mycorrhizal structures.

  • Isolation of spores of VAM fungi: isolation techniques, stain by Melzer's reagent, sample preparation, spore description and fungal species determination.

  • Mycorrhizal extraradical mycelium: extraction techniques (membrane filtration and inserted membrane technique), vital staining techniques, assessment of extraradical hyphal length (grid-line intersect method and image analysis).

  • Long-term cultivation experiment: the aim of the experiment vary with every course run.

Literature
  • NORRIS, JR, DJ READ and AK VARMA. Techniques for the study of mycorrhiza, Part 1. London: Academic Press, 1991. info
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Teacher's information
http://elanor.sci.muni.cz/~mykorrhi
The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.