PřF:Bi6150c Mycorrhizal symbioses - pr. - Course Information
Bi6150c Mycorrhizal symbioses - practice
Faculty of ScienceSpring 2025
- Extent and Intensity
- 0/1/0. 1 credit(s). Type of Completion: z (credit).
In-person direct teaching - Teacher(s)
- prof. RNDr. Miroslav Vosátka, CSc. (seminar tutor)
- Guaranteed by
- prof. RNDr. Miroslav Vosátka, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vít Gloser, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science - Prerequisites (in Czech)
- NOW( Bi6150 Mycorrhizal symbioses )
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Experimental Plant Biology (programme PřF, B-EMB)
- Phycology and Mycology (programme PřF, N-BOT)
- Course objectives
- The aim of the course is to famaliarize students with basic methods of mycorrhizal symbioses research.
- Learning outcomes
- At the end of this practical course, students are able to:
distinguish mycorrhizal symbioses on the base of their morphology and anatomy;
evaluate the effects of mycorrhizal symbiosis on plant growth;
quantify the extent of root colonization and external mycelia growth;
cultivate and propagate the mycorrhizal fungi. - Syllabus
- Mycorrhizal colonization of plant roots: commonly used methods, assessment of root colonization (chemical and microscopic methods), observation of typical mycorrhizal structures.
- Isolation of spores of arbuscular and orchid fungi: isolation techniques, staining by Melzer's reagent, sample preparation, spore description and fungal species determination.
- Mycorrhizal extraradical mycelium: extraction techniques (membrane filtration and inserted membrane technique), vital staining techniques, assessment of extraradical hyphal length (grid-line intersect method and image analysis).
- Long-term cultivation experiment: the aim of the experiment vary with every course run.
- Literature
- GRYNDLER, Milan, Milan BALÁŽ, Hana HRŠELOVÁ, Jan JANSA and Miroslav VOSÁTKA. Mykorhizní symbióza. O soužití hub s kořeny rostlin (Mycorrhizal symbiosis. On the coexistence of fungi with plant roots.). 1. vydání. Praha: Academia, 2004, 366 pp. 1. vydání. ISBN 80-200-1240-0. info
- NORRIS, JR, DJ READ and AK VARMA. Techniques for the study of mycorrhiza, Part 1. London: Academic Press, 1991. info
- Teaching methods
- Practical laboratory course.
- Assessment methods
- Active participation at the course, elaboration of protocols.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
The course is taught: in blocks. - Teacher's information
- http://elanor.sci.muni.cz/~mykorrhi
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2025/Bi6150c