Bi2050 Principles of General Microbiology

Faculty of Science
Spring 2009
Extent and Intensity
3/0/0. 3 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Zdeněk Hubálek, DrSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Hubálek, DrSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: RNDr. Danuška Horáková, CSc.
Timetable
Thu 9:00–11:50 Bpt,01013
Prerequisites
The course is intended for Bachelor students of Cell and Molecular Diagnostics. Prerequisite: exams in Chemistry Basics, and Microscopical Techniques.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Structure and function of microbial cell. Effect of environmental factors on microbial cells. Cell cycle and growth of microorganisms. Microbial metabolism and its regulation. Basics of microbial genetics. Mutations. Recombinations. Plasmids. Soil and water as microbial habitats. Relationships between microorganisms. Microbial diseases. Introduction in general virology. Virion structure. Reproduction of animal, plant and microbial viruses. Virus diseases. Practical exercises: 1.Safety in the microbiological laboratory. 2.Cultivation media and their preparation. 3. Aseptic techniques, inoculation and maintenance of microorganisms. 4.Microscopy of bacteria, Gram staining. 5.Determination of the number of bacterial cells by plate technique. 6.Bacteria in water. 7.Identification of bacteria - introduction. 8. Determination of biologically active substances, sensitivity to antibiotics. 9.Titration of bacteriophage. 10.Microorganisms in soil. 11.Microscopy of the soil microorganisms. Inoculation of micromycetes. 12. Morphology of micromycetes. 13.Yeasts, test of vitality. Main aim is to learn basics of microbiology, including virology.
Syllabus
  • Structure and function of microbial cell.
  • Effect of environmental factors on the microbial cell.
  • Cell cycle and growth of microorganisms.
  • Nutrition and metabolism, regulation of metabolism.
  • Basics of heredity in microorganisms.
  • Mutationse and mutagens.
  • Genetic recombination.
  • Plasmids.
  • Soil and water as habitats of mikrobes.
  • Relationships among microorganisms.
  • Diseases caused by microorganisms.
  • Introduction to general virology.
  • Structure of virus particles (morphology, chemical composition).
  • Reproduction of animal, plant and microbial viruses.
  • Virus diseases.
  • Practical exercises:
  • 1. Safety in microbiological laboratory.
  • 2. Preparation of nutrient microbiological media.
  • 3. Aseptic work, inoculation and maintenance of microorganisms.
  • 4. Microscopy of bacteria, Gram staining.
  • 5. Estimation of bacterial numbers with dilution plate technique.
  • 6. Bacteria in water.
  • 7. Introduction to identification of bacteria.
  • 8. Estimation of biologically active compounds, testing sensitivity to antibiotics.
  • 9. Titration of phage lysate.
  • 10. Microorganisms in soil.
  • 11. Mikroscopy of soil microorganisms. Inoculation of microfungi.
  • 12. Morphology of microfungi.
  • 13. Yeasts - vital test.
Literature
  • NĚMEC, Miroslav and Dana HORÁKOVÁ. Základy mikrobiologie pro učitelské studium. 2. vyd. Brno: Masarykova univerzita v Brně, 1993, 233 s. ISBN 8021008172. info
  • JANDOVÁ, Božena and Ludmila KOTOUČKOVÁ. Praktikum z mikrobiologie. 1. vyd. Brno: Vydavatelství MU, 1996, 67 pp. ISBN 80-210-1374-5. info
  • ROSYPAL, Stanislav. Obecná bakteriologie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1981, 749 s. info
Assessment methods
Lecture once a week, practical exercises with credit, oral examination.
Language of instruction
Czech
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, spring 2012 - acreditation.
  • Enrolment Statistics (Spring 2009, recent)
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