C4840 Methods for labelling and immobilization of biomolecules

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Thu 12:00–13:50 C05/114
Prerequisites
basic knowledge of biochemistry and chemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Modification of biomolecules based on conjugation reactions, introduction of labels and immobilization on surface of polymers and sensing surfaces is used in many current research procedures, bioanalytical assays and therapeutic methods. Initially, the suitable surface target groups of proteins, nucleic acids and saccharides will be mentioned. Next, suitable reagents for conjugation and labelling (enzymes, fluorophores, biotinylation, liposomes, nanoparticles). Methods for activation of surfaces for binding of biomolecules. Characterisation of bioconjugates. Typical applications (hapten-carrier conjugates, labelled antibodies, affinity chromatography, immobilised enzymes, protein and DNA biochips.
Syllabus
  • 1. Modification of proteins and peptides, side chains of amino acids, prosthetic groups.
  • 2. Modification of nucletides, DNA, RNA. Modification of (poly)saccharides, glycoconjugates.
  • 3. Insertion of additional reactive groups into biomolecules. Basic chemical reactions for conjugation.
  • 4. Bioconjugations, homo/heterobifunctional reagents. Cleavable links.
  • 5. Fluorescent labels, fluoresceins, rhodamins, coumarins, FRET.
  • 6. Biotinylation, chelates, oligohistidine tags, boronate complexes. Radiolabels, nanoparticles, liposomes.
Literature
  • HERMANSON, Greg T. Bioconjugate techniques. San Diego: Academic Press, 1995, xxv, 785 s. ISBN 0-12-342336-8. info
Assessment methods
lectures, oral examination
Language of instruction
Czech
Further Comments
Study Materials
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2009, recent)
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