Bi6120c Plant Cell, Tissue and Organ Culture - practical course

Faculty of Science
Spring 2010
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (seminar tutor)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Věra Faronová
Timetable of Seminar Groups
Bi6120c/01: Wed 14:00–15:50 C02/337, J. Dubová
Bi6120c/02: Wed 16:00–17:50 C02/337, J. Dubová
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Passing through this course, students are able to:
apply basic methods and techniques of plant cell, tissue and organ culture in vitro;
propose in vitro methods suitable for plant multiplication, breeding and production;
estimate efficiency of the selected method for specific plant material on the base of visible symptoms.
Syllabus
  • Conditions of in vitro Culture, Laboratory Design.
  • Media Preparation and Sterilization.
  • In vitro Vegetative Propagation: Micropropagation by Proliferation of Axillary Buds, Adventitious Shoot Proliferation; Initiation of Primary Culture, Subculture.
  • Callus Culture: Callus Initiation and Maintenance, Subculture, Organogenesis from Callus.
  • Meristem Culture: Apical and Axillary Meristem Isolation and Culture.
  • Embryo Culture: Isolation and Culture of Young and Mature Zygotic Embryos.
  • Cryopreservation: Alginate Encapsulation/Dehydration of izolated axillary potatoe meristems.
  • Fungi Culture in vitro: Suspension Culture of Wood-Destroying Fungi.
  • Indexing and Certification of in vitro Cultures
Literature
  • KOVÁČ, Jaroslav. Explantátové kultury rostlin. 1. vyd. Ústí nad Labem: Univerzita Jana Evangelisty Purkyně, fakulta pedagogická, 1992, 146 s. ISBN 80-7044-036-8. info
  • ŠEBÁNEK, Jiří and Zdeněk SLADKÝ. Biotechnologie rostlinných explantátů. 1. vyd. Brno: Vysoká škola zemědělská, 1988, 100 s. info
  • PROCHÁZKA, Stanislav and Jiří ŠEBÁNEK. Regulátory rostlinného růstu. Vyd. 1. Praha: Academia, 1997, 395 s. ISBN 8020005978. info
  • NOVÁK, František Jindřich. Explantátové kultury a jejich využití ve šlechtění rostlin. 1. vyd. Praha: Academia, 1990, 208 s. ISBN 8020003444. info
  • SEMAN, Ivan. Biotechnologické metódy v šl'achtení pol'ných plodín. 1. vyd. Bratislava: Príroda, 1990, 271 s. ISBN 80-07-00237-5. info
  • GAMBORG, O. L. and G. C PHILLIPS. Plant Cell, Tissue and Organ Culture, Fundamental Methods (Plant Cell, Tissue and Organ Culture). Berlin, Heidelberg: Springer, 1995, 385 pp. info
  • PIERIK, R. L. M. In vitro culture of higher plants. Dordrecht: Martinus Nijhoff Publishers, 1987. ISBN 90-247-3531-9. info
  • In vitro embryogenesis in plants. Edited by Trevor A. Thorpe. Dordrecht: Kluwer Academic Publishers, 1995, ix, 558 s. ISBN 0-7923-3149-4. info
  • ONDŘEJ, Miloš. Genové inženýrství kulturních rostlin. 1. vyd. Praha: Academia, 1992, 232 s. ISBN 80-200-0310-X. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
  • SLATER, Adrian, Nigel W. SCOTT and Mark R. FOWLER. Plant biotechnology : the genetic manipulation of plants. 1st publ. New York: Oxford University Press, 2003, xxii, 346. ISBN 0199254680. info
  • Phytohormones in plant biotechnology and agriculture : proceedings of the NATO-Russia Workshop held in Moscow, 12-16 May 2002. Edited by Ivana Macháčková - Georgy A. Romanov. Dodrecht: Kluwer Academic Publishers, 2003, xiv, 265. ISBN 1402017235. info
  • Plant biotechnology : current and future applications of genetically modified crops. Edited by Nigel G. Halford. Chichester: John Wiley & Sons, 2006, xii, 303. ISBN 0470021810. info
Teaching methods
Practical course in laboratory of in vitro cultures.
Assessment methods
Protocols of experiments, credit.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2010, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2010/Bi6120c