PřF:Bi8141 Molecular Physiology of Genom - Course Information

Bi8141 Molecular Physiology of Genom

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Stanislav Kozubek, DrSc. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science

Timetable

Tue 8:00–9:50 BFU

Prerequisites

Lectures are attractive for students that are interested in chromatin biology.

Course Enrolment Limitations

The course is offered to students of any study field.

Course objectives

The main aims of lectures from "Molecular Physiology of the Genome" lead to understanding of correlations between structure and function of chromatin. At the end of course, students should be able to understand the basic principles of higher order chromatin structure in the view of nuclear processes such as replication, transcription, splicing and DNA repair. Moreover, students should understand on how chromatin architecture is changed during pathophysiological process such as tumor cell transformation. Lectures from "Molecular Physiology of the Genome" pay an attention to epigenetic regulation of genome, including DNA methylation and histone modifications. Students should be able to explain relationship between histone signature and gene expression. Taken together, the chromatin biology is explained from the view of basic research and students should be familiarized with recent trends in this attractive filed of science.

Syllabus

• 1. Cell nucleus, nucleolus – compartmentalization of interphase nuclei
• 2. Transcription, RNA processes and RNA interference
• 3. Structure of chromatin and transcription
• 4. Structure of chromatin and cell differentiation
• 5. Structure of chromatin and apoptosis
• 6. Epigenetic aspects of chromatin
• 7. Cytoskeleton, lamins and controversial nuclear matrix
• 8. BAC/PAC libraries and DNA probe preparation for FISH technique
• 9. Confocal microscopy and related methods
• 10. Structure of chromatin and epigenetic changes in tumor cells
• 11. High resolution cytometry – principles, statistic and bioinformatic analysis
• 12. Microarray technology
• 13. DNA reparation mechanisms
• 14. Credit week - J.G. Mendel and his importance, Mendelianum excursion
• Visit - http://www.ibp.cz/labs/LMCC/publications.php

Literature

• GAŇOVÁ, Alena, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK, Michal KOZUBEK, Raisa GOVORUN, Irena KOUTNÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavla JIRSOVÁ, Renata PASEKOVÁ and Evgenij KRASAVIN. Exchange aberrations among 11 chromosomes of human lymphocytes induced by gamma-rays. International Journal of Radiation Biology. 2001, vol. 77, No 4, p. 419-429. ISSN 0955-3002. abstract info
• BÁRTOVÁ, Eva, Stanislav KOZUBEK, Pavla JIRSOVÁ, Michal KOZUBEK, Hana GAJOVÁ, Emilie LUKÁŠOVÁ, Magdalena SKALNÍKOVÁ, Alena GAŇOVÁ, Irena KOUTNÁ and Michael HAUSMANN. Nuclear structure and gene activity in human differentiated cells. Journal of Structural Biology. San Diego,USA: Academic Press, 2002, vol. 139, No 2, p. 76-89. ISSN 1047-8477. info
• KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Andrea MAREČKOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ and Adriana JERGOVÁ. High-resolution cytometry of FISH dots in interphase cell nuclei. Cytometry. New York: International Society for Analytical Cyt, 1999, vol. 36, No 4, p. 279-293. ISSN 0196-4763. info
• JANSOVÁ, Eva, Petr KRONTORÁD, Zbyněk SVOBODA, Tomáš PAVLÍK, Irena KOUTNÁ, Michal KOZUBEK, Jan ŽALOUDÍK and Stanislav KOZUBEK. Nové možnosti v diagnostice kolorektálního karcinomu s využitím technologie cDNA mikročipů (New possibility in colorectal carcinoma diagnosis using cDNA microarrays). Klinická onkologie. Brno: ApS Brno, spol.s.r.o., 2004, 6/17, No 6, p. 203-207. ISSN 0862-495X. info
• ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Pavel MATULA, Petr MATULA and Michal KOZUBEK. Dynamics of HP1 transgene loci movement and silencing in living cells. In Biophysics of the Genome. First Edition 2004. Brno: Masaryk University, 2004, p. 66-71. ISBN 80-210-3560-9. info
• FALK, Martin, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK and Michal KOZUBEK. Topography of genetic elements of X-chromosome relative to the cell nucleus and to the chromosome X territory determined for human lymphocytes. Gene. Amsterdam: Elsevier, 2002, vol. 292, No 1, p. 13-24. ISSN 0378-1119. info
• BÁRTOVÁ, Eva, Stanislav KOZUBEK, Hana GAJOVÁ, Pavla JIRSOVÁ, Jitka ŽLŮVOVÁ, Renata TASLEROVÁ, Irena KOUTNÁ and Michal KOZUBEK. Cytogenetics and cytology of retinoblastomas. J Cancer Res. Clin.Oncol. Berlin: Springer-Verlag, 2003, vol. 129, No 2, p. 89-99. ISSN 0171-5216. info

Teaching methods

Theoretical study by means of literature provided by lecturer, lectures, and internet resources. Student is supposed to analyse, present and discuss a scientific paper.

Assessment methods

Oral exam, each student prepares analysis of scientific paper in selected field of science or recommended by tutor. Courses must be attended. Tutor’s presentation will be provided in pdf format.

Language of instruction

Czech

Follow-Up Courses

• Bi9393 Analytical cytometry

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

Teacher's information

http://www.ibp.cz/

• Enrolment Statistics (Spring 2011, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2011/Bi8141