Bi8141 Molecular Physiology of Genom

Faculty of Science
Spring 2021
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Stanislav Kozubek, DrSc. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
Guaranteed by
prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Lectures are attractive for students that are interested in chromatin biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aims of lectures from "Molecular Physiology of the Genome" lead to understanding of correlations between structure and function of chromatin. At the end of course, students should be able to understand the basic principles of higher order chromatin structure in the view of nuclear processes such as replication, transcription, splicing and DNA repair. Moreover, students should understand on how chromatin architecture is changed during pathophysiological process such as tumor cell transformation. Lectures from "Molecular Physiology of the Genome" pay an attention to epigenetic regulation of genome, including DNA methylation and histone modifications. Students should be able to explain relationship between histone signature and gene expression. Taken together, the chromatin biology is explained from the view of basic research and students should be familiarized with recent trends in this attractive filed of science.
Learning outcomes
After finishing this course students will be able to explain:
- correlations between structure and function of chromatin
- the basic principles of higher order chromatin structure in the view of nuclear processes such as replication, transcription, splicing and DNA repair
- how chromatin architecture is changed during pathophysiological process such as tumor cell transformation
- the epigenetic regulation of genome, including DNA methylation and histone modifications
- relationship between histone signature and gene expression
Syllabus
  • 1. Cell nucleus, nucleolus – compartmentalization of interphase nuclei
  • 2. Transcription, RNA processes and RNA interference
  • 3. Structure of chromatin and transcription
  • 4. Structure of chromatin and cell differentiation
  • 5. Structure of chromatin and apoptosis
  • 6. Epigenetic aspects of chromatin
  • 7. Cytoskeleton, lamins and controversial nuclear matrix
  • 8. BAC/PAC libraries and DNA probe preparation for FISH technique
  • 9. Confocal microscopy and related methods
  • 10. Structure of chromatin and epigenetic changes in tumor cells
  • 11. High resolution cytometry – principles, statistical and bioinformatic analysis
  • 12. Microarray technology
  • 13. DNA reparation mechanisms
  • 14. Credit week - J.G. Mendel and his importance, Mendelianum excursion
  • Visit - http://www.ibp.cz/labs/LMCC/publications.php
Literature
Teaching methods
Theoretical study by means of literature provided by lecturer, lectures, and internet resources. Student is supposed to analyse, present and discuss a scientific paper.
Assessment methods
Oral exam, each student prepares analysis of scientific paper in selected field of science or recommended by tutor. Courses must be attended. Tutor’s presentation will be provided in pdf format.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught once in two years.
Information on the per-term frequency of the course: Předmět se vypisuje každý lichý rok.
The course is taught: every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
Teacher's information
http://www.ibp.cz/
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2016, spring 2018, Spring 2019, Spring 2024.
  • Enrolment Statistics (Spring 2021, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2021/Bi8141