S2006 Advanced methods of biophysics in experimental biology

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Ctirad Hofr, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Course Enrolment Limitations
The course is offered to students of any study field.
Syllabus
  • Definition and characteristics of absorption, fluorescence and phosphorescence Instrumentation - spectrophotometer, spectrofluorometer Steady-state fluorescence Time-resolved fluorescence Fluorescence anisotropy and its change after interaction of macromolecules Absorption spectroscopy in biological analysis of molecules Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules Intrinsic fluorescence of biologically important molecules Extrinsic fluorescence – fluorescent labels, probes, and indicators Fluorescence labeling of DNA and proteins Fluorescence microscopy Analytical applications of fluorescence for concentration determination Examples of fluorescence application in biology
Literature
  • LAKOWICZ, Joseph R. Principles of fluorescence spectroscopy. 3rd ed. New York: Springer, 2006, xxvi, 954. ISBN 0387312781. info
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019.