PřF:S2006 Fluorescence methods - Course Information

S2006 Fluorescence methods in life sciences – A journey from a molecule to cells

Extent and Intensity

2/0. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)

Guaranteed by

doc. Mgr. Ctirad Hofr, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 11. 5. 9:00–11:50 E35/2S087, Tue 12. 5. 9:00–11:50 E35/2S087, Wed 13. 5. 9:00–11:50 E35/2S087, Thu 14. 5. 9:00–11:50 E35/2S087, Fri 15. 5. 9:00–11:50 E35/2S087

Course Enrolment Limitations

The course is offered to students of any study field.

Syllabus

• Definition and characteristics of absorption, fluorescence and phosphorescence Instrumentation - spectrophotometer, spectrofluorometer Steady-state fluorescence Time-resolved fluorescence Fluorescence anisotropy and its change after interaction of macromolecules Absorption spectroscopy in biological analysis of molecules Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules Intrinsic fluorescence of biologically important molecules Extrinsic fluorescence – fluorescent labels, probes, and indicators Fluorescence labeling of DNA and proteins Fluorescence microscopy Analytical applications of fluorescence for concentration determination Examples of fluorescence application in biology

Literature

• LAKOWICZ, Joseph R. Principles of fluorescence spectroscopy. 3rd ed. New York: Springer, 2006, xxvi, 954. ISBN 9781489978806. info

Language of instruction

English

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

• Enrolment Statistics (Spring 2015, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2015/S2006