C5993 New Developments in Capillary Electrophoresis with focus on Bioanalysis

Přírodovědecká fakulta
jaro 2013
Rozsah
2/0/0. 2 kr. (plus ukončení). Doporučované ukončení: zk. Jiná možná ukončení: k.
Vyučující
Christian Nilsson, PhD. (přednášející)
prof. Mgr. Jan Preisler, Ph.D. (přednášející)
Garance
prof. Mgr. Jan Preisler, Ph.D.
Ústav chemie – Chemická sekce – Přírodovědecká fakulta
Dodavatelské pracoviště: Ústav chemie – Chemická sekce – Přírodovědecká fakulta
Rozvrh
Po 14:00–15:50 C14/207
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
Cíle předmětu
The course will provide an in depth description of new development in capillary electrophoresis. After a more general introduction, various aspects of capillary electrophoresis and its applications will be covered. The main focus will be on bioanalytical applications where the technology is applied at the companies and at universities. One important direction in capillary electrophoresis is the development of microchip-based technologies. Such technologies could, for example, be utilized for portable diagnostic devices, as well as other applications when analysis is needed outside the laboratory.
Osnova
  • Introduction CE, Electroosmotic flow, Electrophoretic mobility, Factors affecting electrophoretic migration, Historical Development, Instrumentation, Injection, Detection
  • CE modes, Capillary Zone electrophoresis, Capillary Gel Electrophoresis, Capillary Isotachophoresis, Capillary Isoelectric focusing, Micellar electrokinetic Chromatography, Capillary Electrochromatography, Additives for CE
  • Capillary Electrochromatography, Column technology, Packed capillaries, Monolithic column capillaries, Open-tubular capillaries, Nanoparticle-based CEC
  • Injection for CE, Sample preconcentration, On-column concentration, Sample stacking, Sweeping
  • Detection for CE, Improvement of detection sensitivity, UV, LIF, Mass spectrometry, Electrochemical detection
  • Applications of CE, Small molecules, Amino Acids, Peptides, DNA, Proteins, Chiral separation, Cyclodextrin, DNA sequencing, SDS-Proteins, Analysis of Monoclonal antibodies, Carbonhydrate analysis, Cell analysis, 2D-CE, Affinity CE
  • Protein separation, Capillary coating, Dynamic coating, Covalent coating
  • Microchip-based electrophoresis, Microchip-based separation, Microchip-based methods
  • Microchip fabrication, Injection, Detection, LIF, Electrochemical detection, MS coupling
  • Applications of microchip electrophoresis, DNA analysis, protein analysis, Cell analysis, Portable CE for diagnostics
Výukové metody
Lectures. Each week lecture notes and complementary material will be used as course material.
Metody hodnocení
Written examination
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je vyučován jednorázově.
  • Statistika zápisu (nejnovější)
  • Permalink: https://is.muni.cz/predmet/sci/jaro2013/C5993