C5993 New Developments in Capillary Electrophoresis with focus on Bioanalysis

Faculty of Science
Spring 2013
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
Christian Nilsson, PhD. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Mon 14:00–15:50 C14/207
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course will provide an in depth description of new development in capillary electrophoresis. After a more general introduction, various aspects of capillary electrophoresis and its applications will be covered. The main focus will be on bioanalytical applications where the technology is applied at the companies and at universities. One important direction in capillary electrophoresis is the development of microchip-based technologies. Such technologies could, for example, be utilized for portable diagnostic devices, as well as other applications when analysis is needed outside the laboratory.
Syllabus
  • Introduction CE, Electroosmotic flow, Electrophoretic mobility, Factors affecting electrophoretic migration, Historical Development, Instrumentation, Injection, Detection
  • CE modes, Capillary Zone electrophoresis, Capillary Gel Electrophoresis, Capillary Isotachophoresis, Capillary Isoelectric focusing, Micellar electrokinetic Chromatography, Capillary Electrochromatography, Additives for CE
  • Capillary Electrochromatography, Column technology, Packed capillaries, Monolithic column capillaries, Open-tubular capillaries, Nanoparticle-based CEC
  • Injection for CE, Sample preconcentration, On-column concentration, Sample stacking, Sweeping
  • Detection for CE, Improvement of detection sensitivity, UV, LIF, Mass spectrometry, Electrochemical detection
  • Applications of CE, Small molecules, Amino Acids, Peptides, DNA, Proteins, Chiral separation, Cyclodextrin, DNA sequencing, SDS-Proteins, Analysis of Monoclonal antibodies, Carbonhydrate analysis, Cell analysis, 2D-CE, Affinity CE
  • Protein separation, Capillary coating, Dynamic coating, Covalent coating
  • Microchip-based electrophoresis, Microchip-based separation, Microchip-based methods
  • Microchip fabrication, Injection, Detection, LIF, Electrochemical detection, MS coupling
  • Applications of microchip electrophoresis, DNA analysis, protein analysis, Cell analysis, Portable CE for diagnostics
Teaching methods
Lectures. Each week lecture notes and complementary material will be used as course material.
Assessment methods
Written examination
Language of instruction
English
Further Comments
Study Materials
The course is taught only once.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2013/C5993