Bi7410 Protein Engineering

Faculty of Science
Spring 2014
Extent and Intensity
1/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Radka Chaloupková, Ph.D. (lecturer)
Mgr. Eva Šebestová, Ph.D. (lecturer)
Mgr. Jan Brezovský, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Radka Chaloupková, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 16:00–17:50 B11/235
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
The aim of this course is to introduce methods and strategies commonly used in protein engineering.
At the end of the course, students should be able to understand and explain differences between rational design and directed evolution, and have a general knowledge about miscellaneous topics such as searches in bioinformatics databases, isolation, expression or purification of novel proteins. Students will also get an overview of several biophysical techniques used for analysis of secondary, tertiary and quaternary structure, as well as of screening methods used for selection of novel protein variants with improved properties.
Syllabus
  • 1. Protein synthesis, protein structure, protein function and structure-function relationships.
  • 2. Identification of putative enzymes in sequence databases, bioinformatic analysis.
  • 3. Isolation of genes from host organisms, cloning, preparation of recombinant proteins, host organisms, protein expression and protein purification.
  • 4. Structural characterization of proteins, an overview of spectroscopic techniques for the analysis of protein secondary and tertiary structure; an overview of techniques for analysis of protein quaternary structure.
  • 5. Enzymes, enzyme catalysis, factors influencing the speed of enzymatic reaction.
  • 6. Enzyme applications, targets of protein engineering, protein engineering approaches, advantages and limitations.
  • 7. Rational design, prediction of the structure of enzyme variant, evaluation of the effect of mutations on enzyme structure and function.
  • 8. Directed evolution, screening of mutants.
  • 9. Examples of application of protein engineering to improve enzyme catalytic efficiency.
  • 10. Examples of application of protein engineering to improve enzyme stability.
  • 11. Examples of application of protein engineering to improve enzyme enantioselectivity.
Literature
    recommended literature
  • Protein engineering handbook. Edited by Stefan Lutz - Uwe Bornscheuer. Weinheim: Wiley-VCH, 2009, xli, 409-9. ISBN 9783527318506. info
    not specified
  • Directed evolution library creation : methods and protocols. Edited by Frances Hamilton Arnold - George Georgiou. Totowa, N.J.: Humana Press, 2003, x, 224. ISBN 1588292851. info
  • FERSHT, Alan. Structure and mechanism in protein science :a guide to enzyme catalysis and protein folding. New York: W.H. Freeman, 1998, xxi, 631 s. ISBN 0-7167-3268-8. info
Teaching methods
Lectures, class discussion.
Assessment methods
Final written test consists of 25 questions and is scored on a 25-point scale. A minimum score of 13 is required to successfully pass the exam.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2014, recent)
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