Bi8313 Genetic Engineering - Laboratory Course

Faculty of Science
Spring 2017
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Mgr. Ivana Mašlaňová, Ph.D. (seminar tutor)
Mgr. Pavol Bárdy, Ph.D. (seminar tutor)
Mgr. Michal Zeman, Ph.D. (seminar tutor)
Mgr. Martin Benešík, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Wed 9:00–12:50 E25/119, Thu 14:00–15:50 E25/119
Prerequisites
! B6492 Molecular biology - practice 2 && ( Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology ) && ( Bi6405 Molecular Methods - practice || B6405 Molecular Methods - practice ) && NOW( Bi8090 Gene engineering ) && ! Bi5491 Molecular Diagnostics
Basic methods of molecular biology (lecture and practice).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 28 student(s).
Current registration and enrolment status: enrolled: 0/28, only registered: 0/28
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to perform methods of DNA cloning, choose suitable vectors and restriction endonucleases for DNA cloning, preparation of molecular probes, design primers and use polymerase chain reaction for modification of DNA ends and prepare DNA samples for sequencing.
Syllabus
  • 1. Isolation of Sau3AI and Sau96I restriction endonucleases.
  • 2. Cloning in pUC plasmid vectors (dephosphorylation of DNA ends by alkaline phosphatase, ligation of vector and foreign DNA by T4 DNA ligase, transformation of competent cells, identification of recombinant clones on ampicillin plates, restriction analysis and construction of restriction maps of recombinant plasmids).
  • 3. DNA hybridization (non-radioactive labeling of DNA by digoxigenin, Southern blotting, hybridization with labeled probe, immunologic detection).
  • 4. Modification of DNA ends by PCR, cloning of PCR products.
  • 5. DNA sequencing.
Literature
  • BROWN, T. A. Klonování genů a analýza DNA : úvod. Translated by Martin Fellner. 1. české vyd. V Olomouci: Univerzita Palackého, 2007, xviii, 389. ISBN 9788024417196. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 1st ed. Brno: Masarykova univerzita, 2005, 194 pp. 1. vydání. ISBN 80-210-3841-1. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Teaching methods
The course is taught in six 4 h blocks in research molecular biology laboratories of the department. Students perform practical experiments according to the instructions of teacher and according to printed manuals.
Assessment methods
The course is completed after work out an elaborate and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Studenti kteří již absolvovali B6492 tento předmět nezapisují z důvodu stejného obsahu. Prosíme striktně o dodržování limitu počtu studentů.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2017, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2017/Bi8313