C4050 Analytical Chemistry II

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Přemysl Lubal, Ph.D. (lecturer)
prof. Mgr. Jan Preisler, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Přemysl Lubal, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Wed 8:00–9:50 B11/205
Prerequisites
C3100 Analytical Chemistry I && C3110 Analytical Chemistry I-sem.
The prerequisite is passing of subjects Analytical chemistry I (C3100), Physical chemistry I (C3140), General chemistry (C1020), Inorganic chemistry I (C1061), Inorganic chemistry II (C2062), Organic chemistry I (C2021), Organic chemistry II (C3022) and relevant Laboratory courses from those subjects.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
A systematic treatment of the basic principles of analytical chemistry for students of chemistry - part II. The course includes electroanalytical, optical and separation methods. On the base of acquired knowledge student is able to understand basic principles of instrumental analytical chemistry. Utilizing informations from the course, students can also analyse problems and propose solutions.
Syllabus
  • 1. Introduction into subject instrumental analytical chemistry. Evaluation of results of analysis - statistics and GLP knowledge. Physical properties and analytical signal, relationship of analytical signal and concentration, calibration curves and their evaluation, standardisation of relative physical methods. Parameters of analytical method (detection limit, limit of determination, sensitivity, robustness, precision, accuracy, etc.). Rejection of outliers, circle test.
  • 2. Chosen electroanalytical methods. Potentiometric methods, basic definitions and terms. Indicator and reference electrodes. pH determination. Potentiometric indication of course of titration and equivalence point. Acid-base, redox, precipitation, complexometric titrations with potentiometric indication. Potentiometric evaluation of equivalence point (Gran linearization of titration curve).
  • 3. Conductometric methods - basic definitions and terms. Direct conductometry, application for conductometric determination of equivalence point.
  • 4. Electrogravimetry, coulometry - basic definitions and terms. Polarisation curves, deposition current, faradaic current. Electrolysis under constant potential and constant current. Electrolytic separation of metals, determination of copper and silver. Calculations of potentials for deposition, coulometry under constant current. Coulometric titrations (absolute titration methods).
  • 5. Voltammetry, polarography - basic definition and terms. Polarographic analysis (quantitative and qualitative analysis). Examples of applications and determinations. Amperometric, biamperometric and bipotentiometric titrations. Karl-Fischer determination of water.
  • 6. Chosen optical methods. Introduction - description of electromagnetic radiation, basic definition and terms, relationships (e.g. Bouger - Lambert-Beerův law), origin of absorption and emission of light. Division of optical analytical methods. It is necessary to know the light sources, dispersion elements, detectors, methodology.
  • 7. Molecular absorption spectroscopy (UV, VIS, IR). Molecular scattering spectroscopy (turbidimetry and nephelometry). Luminescent methods.
  • 8. Atomic absorption and emission spectroscopy.
  • 9. Chosen separation methods. Solvent extraction - basic terms and definition. Extraction equilibria in two-phase system. Solvate extraction, ionic and nonionic substances. Analytical use of ion exchangers - basic terms and definition. Solid and liquid ion exchangers - characteristic, relationships and examples of the applications.
  • 10. Chromatography on thin layer of sorbent (thin layer chromatography, paper chromatgraphy) - principles and examples of applications. Gas analysis - classic (principle and examples of applications), gas chromatography (theory, basic description of instrumentation, qualitative and quantitative analysis, examples of analytical applications).
  • 11. HLPC - high performance chromatography (theory, basic description of instrumentation, qualitative and quantitative analysis, examples of analytical applications).
  • 12. Electromigration methods (zone electrophoresis, electrophoresis on carriers and isotachophoresis} - basic definitions and terms, kinds of methods, basic description of instrumentation, examples of analytical applications). Mass spectrometry.
Literature
  • Sommer L a kol., Základy analytické chemie II, VUTium Brno 2000.
  • Analytická příručka. Edited by Jaroslav Zýka. 4., upr. vyd. Praha: SNTL - Nakladatelství technické literatury, 1988, 831 s. info
  • SKOOG, Douglas A. Fundamentals of analytical chemistry. 8th ed. Belmont, Calif.: Brooks/Cole, 2004, 1 sv. ISBN 0534417965. info
  • HARRIS, Daniel C. Quantitative chemical analysis. 7th ed. New York: W. H. Freeman and Company, 2007, 1 sv. ISBN 0716776944. info
  • Analytical chemistry : a modern approach to analytical science. Edited by Jean-Michel Mermet - Matthias Otto - M. Valcárcel Cases. 2nd ed. Weinheim: Wiley-VCH, 2004, xxviii, 11. ISBN 3527305904. info
  • SCHWEDT, Georg. The essential guide to analytical chemistry. Translated by Brooks Haderlie. 2nd ed. Chichester: John Wiley & Sons, 1997, xii, 248. ISBN 0471974129. info
  • CHRISTIAN, Gary D. Analytical chemistry. 6th ed. Hoboken, NJ: John Wiley & Sons, 2003, xix, 828 s. ISBN 0-471-21472-8. info
  • Enke Ch., The Art and Science of Chemical Analysis, Wiley New York 2001
Teaching methods
lectures
Assessment methods
2 hour lecture
Knowledge obtained during lectures will be tested by final combined examinanition. The succesful written test is followed by oral exam.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2019, recent)
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