PřF:C9075 Adv. Liq. Chrom. - Course Information

C9075 Advanced liquid chromatography

Extent and Intensity

1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).

Teacher(s)

doc. RNDr. Jiří Urban, Ph.D. (lecturer)

Guaranteed by

doc. RNDr. Jiří Urban, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Jiří Urban, Ph.D.
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science

Timetable

Mon 18. 2. to Fri 17. 5. Thu 13:00–13:50 C14/207

Prerequisites

Understanding of basic principles of chromatography, effect of polarity of compounds and individual functional groups.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Analytical Chemistry (programme PřF, D-CH4)
• Analytical Chemistry (programme PřF, N-CH)

Course objectives

During Spring 2019 the course will be presented in English. The main aim of the course is to present current developments in the field of liquid-based chromatography. The special attention will be paid to individual retention mechanisms, new types of stationary phases, elevated temperature, gradient elution, and multidimensional separations. Part of the course will be focused on miniaturization and unusual detection.
At the end, student will be able understand current liquid chromatography.

Learning outcomes

After the course student will:
- know the most comon separation mechanisms;
- know how to characterize HPLC column;
- know new types of stationary phases;
- apply knowledge about gradient elution, high temperature separations and multidimensional techniques;
- provide orientation in miniturization of separation techniques;
- be able to compare several detection methods applicable in HPLC;
- be able to optimize isocratic and graient elution;

Syllabus

• 1. Retention mechanisms – principle of chromatography, instrumetnation, sumnmary of basic mechanisms (NP, RP, HILIC, IEX, IC, SEC)
• 2. Column characterization – porosity, permeability, efficiency, resolution, kinetic plot
• 3. Silicagel – structrue, modification, fully porous, nonporous, superficially porous particles
• 4. Inorganic monoliths – preparation, modification, application
• 5. Polymer monolilths – classification, preparation, modification, application
• 6. Gradient elution - models, prediction, peak compression, application
• 7. Elevated temperature - entropy, enthalpy, quality of separation, thermal gradients
• 8. Ultra high pressure - analysis time, instrumentation, application
• 9. Multidimensional chromatography – theory, optimization, sample transfer, application
• 10. Miniaturization - nano LC, chips, integration, uTAS
• 11. (Unusual) detection – UV, MS, (elektrochemistry, NMR, IR, …)
• 12. Separation optimization in Excel

Literature

recommended literature
• Aktuální věděcká literatura (Analytical Chemistry, Journal of Chromatography A, ...)
• SNYDER, Lloyd R., J. J. KIRKLAND and John W. DOLAN. Introduction to modern liquid chromatography. 3rd ed. Hoboken: John Wiley & Sons, 2010, xli, 912. ISBN 9780470167540. info
• JANDERA, Pavel and Jaroslav CHURÁČEK. Gradient elutiom in column liquid chromatography : theory and practice. Amsterdam: Elsevier, 1985, 510 s. ISBN 0444416161. info

Teaching methods

The course is presented as weekly presentations focused on individual parts of advanced liquid chrmatography.
At the end of each class, designated time for discussion will be allowed.

Assessment methods

The course is finished with both written and oral exam.
At first, short test with 10 questions is answered. Following oral examination with two questions is allowed only in more than five questions are answered correctly.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

• Enrolment Statistics (Spring 2019, recent)
  
• Permalink: https://is.muni.cz/course/sci/spring2019/C9075