C9075 Advanced liquid chromatography

Faculty of Science
Spring 2023
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Jiří Urban, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jiří Urban, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Contact Person: doc. RNDr. Jiří Urban, Ph.D.
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Mon 8:00–9:50 C14/207
Prerequisites
Understanding of basic principles of chromatography, effect of polarity of compounds and individual functional groups.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main aim of the course is to present current developments in the field of liquid-based chromatography. The special attention will be paid to individual retention mechanisms, new types of stationary phases, elevated temperature, gradient elution, and multidimensional separations. Part of the course will be focused on miniaturization and unusual detection.
At the end, student will be able understand current liquid chromatography.
Learning outcomes
After the course student will:
- know the most comon separation mechanisms;
- know how to characterize HPLC column;
- know new types of stationary phases;
- apply knowledge about gradient elution, high temperature separations and multidimensional techniques;
- provide orientation in miniturization of separation techniques;
- be able to compare several detection methods applicable in HPLC;
- be able to optimize isocratic and graient elution;
Syllabus
  • 1. Retention mechanisms – principle of chromatography, instrumetnation, sumnmary of basic mechanisms (NP, RP, HILIC, IEX, IC, SEC)
  • 2. Column characterization – porosity, permeability, efficiency, resolution, kinetic plot
  • 3. Silicagel – structrue, modification, fully porous, nonporous, superficially porous particles
  • 4. Inorganic monoliths – preparation, modification, application
  • 5. Polymer monolilths – classification, preparation, modification, application
  • 6. Gradient elution - models, prediction, peak compression, application
  • 7. Elevated temperature - entropy, enthalpy, quality of separation, thermal gradients
  • 8. Ultra high pressure - analysis time, instrumentation, application
  • 9. Multidimensional chromatography – theory, optimization, sample transfer, application
  • 10. Miniaturization - nano LC, chips, integration, uTAS
  • 11. (Unusual) detection – UV, MS, (elektrochemistry, NMR, IR, …)
  • 12. Separation optimization in Excel
Literature
    recommended literature
  • Aktuální věděcká literatura (Analytical Chemistry, Journal of Chromatography A, ...)
  • SNYDER, Lloyd R., J. J. KIRKLAND and John W. DOLAN. Introduction to modern liquid chromatography. 3rd ed. Hoboken: John Wiley & Sons, 2010, xli, 912. ISBN 9780470167540. info
  • JANDERA, Pavel and Jaroslav CHURÁČEK. Gradient elutiom in column liquid chromatography : theory and practice. Amsterdam: Elsevier, 1985, 510 s. ISBN 0444416161. info
Teaching methods
The course is presented as weekly presentations focused on individual parts of advanced liquid chrmatography.
At the end of each class, designated time for discussion will be allowed.
Assessment methods
The course is finished with both written and oral exam.
At first, short test with 10 questions is answered. Following oral examination with two questions is allowed only in more than five questions are answered correctly.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2023, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2023/C9075