PřF:C5990 Applied enzymology - Course Information
C5990 Applied enzymology
Faculty of ScienceSpring 2020
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
- Teacher(s)
- prof. RNDr. Petr Skládal, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Petr Skládal, CSc.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Prerequisites
- basic overview of biochemistry
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- An advanced lecture. At the end students will understand the topics related to the practical use of enzymes, including nomenclature and kinetics, preparation and storage methods, use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors.
- Learning outcomes
- Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
- Syllabus
- 1. Initial information on enzymes. Basic terms. Enzyme activity. 2. Methods for measuring enzyme activity - optical and electrochemcial, suitable substrates. Examples of measurement for most important enzymes. 3. Isolation of enzymes, purification procedures, chromatography. Commercial sources of enzymes. Enzyme structure characterisation. 4. Mechanisms of enzyme catalysis. Basic principles, typical examples. 5. Kinetics of enzyme reaction with substrate, parameters vmax (vlim) and Km including methods for determination. Software for enzyme kinetics, examples of use. 6. Reactions with multiple substrates, classification, different mechanisms. Inhibitors, types, inhibition constants, kinetic studies. 7. Effect of external factors on activity (pH, temperature, ionic strength, viscosity). Cooperative effects in enzyme action. 8. Bioanalytical application of enzymes. Enzyme assays in clinical analysis. 9. Enzyme-based biosensors, measuring systems, examples of applications. 10. Immobilization of enzymes, enzyme reactors. Entrapment inside polymers, covalent attachment on beads and sensing surfaces. 11. Enzymes in immunochemical techniques, ELISA. Enzyme labels and methods for preparation of enzyme conjugates. 12. Industrial application of enzymes. Information sources on enzymes on internet.
- Literature
- MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
- Teaching methods
- lecture course, finished by examination
- Assessment methods
- Participation at lectures, oral examination - randon selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2020, recent)
- Permalink: https://is.muni.cz/course/sci/spring2020/C5990